馬伝染性貧血における血清蛋白質の電気泳動分析所見と末梢血のリンパ様細胞の電子顕微鏡的観察

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This study was made to elucidate fundamental nature of chronic equine infectious anemia (EIA) using two horses, infected artificially with chronic EIA. The results obtained as follows. No siderocytes were detected in the peripheral blood of No.28 horse and the immunodiffusion test of the blood was positive. The blood from No.28 horse, infected with chronic EIA inoculated into No.30, a healthy horse. The same procedure was applied to No.31 healthy horse, using the blood from No.30 horse infected with EIA. No.31 horse showed the first signs of pyrexia in 36 days and three times in the course of 387 days after inoculation. This together with the findings of histopathological liver biopsy in 39 days suggests that No.31 horse was infected with EIA. As regards No.31 horse, the immunodiffusion test turned positive in 52 days and siderocytes, together with a number of lymphoid cells, were detected in 37 days, their maximal number amounting to 49 per 10, 000 leucocytes. No.31 horse did not show symptoms of pyrexia over a long period of 213 days since last pyrexia and siderocytes in the peripheral blood disappeared after 183 days, indicating that No.31 horse was infected with typical chronic EIA. Leucocytes were collected from the peripheral blood in which no siderocytes were detectable under an electron microscope. Small round cells were occaionally found. Most of them included locally developed, and vesicularly swelling rough surfaced endoplasmic reticulum (r-ER) (Plate II). Cells containing extremely expanded r-ER of low electron density were rarely observed (Plate III). A small number of cells having a structure similar to Russel body around the concave part of the nucleus were also observed (Plate IV). These cells were clearly distinguishable from lymphocytes and plasma cells by their ultra fine structure and grouped into a category of small lymphoid cells (l-cell). The percentages of Albumin, α_1-globulin (glob.), α_2-glob., β-glob. and γ-glob. in serum protein were 26.O, 9.5, 11.0, 25.5, and 28.0, respectively. γ-Glob. which increased slightly at the early stage of infection, decreased 277 days after inoculation. Ultracentrifugation showed that the percentages of 3.5, 7, 19s in serum protein were 65.40, 29.92, and 4.68, respectively. These cells were considered to be the same as l-cells propagating in the cortex of lymph nodes, expect that the cells differed in size. Viewing the results obtained in this study, chronic type EIA is considered to be leukemic leukosis of l-cells.
帯広畜産大学の論文
1977-01-17

馬伝染性貧血における血清蛋白質の電気泳動分析所見と末梢血のリンパ様細胞の電子顕微鏡的観察

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