An Ultramicro Phosphorimetric Assay for Alkaline and Acid Phosphatase Activities
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概要
- 論文の詳細を見る
A sensitive phosphorimetric method for the assay of alkaline and acid phosphatases in biological materials is described. p-Nitrophenol, formed enzymatically from the substrate p-nitrophenyl phosphate, is extracted with ether and then determined phosphorimetrically in a mixture of ether and ethanolic potassium hydroxide. The method is rapid and very sensitive, and thus requires as little as 0.5-5μl of human serum or 0.1-5μg of protein of rat tissue with a detection limit for p-nitrophenol formed of as little as 10 pmol.
- 公益社団法人日本薬学会の論文
- 1980-10-25
著者
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大倉 洋甫
Faculty Of Pharmaceutical Sciences Kyushu University
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山口 政俊
Faculty Of Pharmaceutical Science Fukuoka University
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大倉 洋甫
Faculty Of Pharmaceutical Science Kyushu University
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宮本 始龍
Faculty of Pharmaceutical Sciences, Kyushu University
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宮本 始龍
Faculty Of Pharmaceutical Sciences Kyushu University
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