High-Performance Liquid Chromatography of Arachidonic Acid Metabolites and Its Application to the Determination of Leukotriene B_4 in Stimulated Leukocytes

概要

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A highly sensitive high-performance liquid chromatography with fluorescence detection for the determination of arachidonic acid metabolites is described. The metabolites are converted into corresponding fluorescent derivatives by reaction with 3-bromomethyl-6,7-methylenedioxy-1-methyl-2(1H)-quinoxalinone in the presence of potassium hydrogen carbonate and 18-crown-6 in acetonitrile. The derivatives are separated on a reversed-phase column (Inertsil ODS) with aqueous acetonitrile and detected fluorimetrically. The detection limits are 5-15 fmol at a signal-to-noise ratio of 3 in a 10-μl injection volume. The method is applied to the determination of leukotriene B_4 produced in stimulated leukocytes.
社団法人日本薬学会の論文
1989-10-25