光触媒酸化反応を利用する5-ヒドロキシインドール類のプレカラム蛍光誘導体化液体クロマトグラフィー(<特集>流れを利用する新しい分析技術)

概要

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We have developed a fluorescence derivatization method of 5-hydroxyindoles (serotonin, 5-hydroxyindole-3-acetic acid, N-acetylserotonin) based on benzylamine derivatization, followed by photocatalytic oxidation. In the present study, a photocatalyst, which was titanium dioxide supported on a diatomaceous earth particle, was used instead of potassium hexacyanoferrate(III) as a chemical oxidizing agent employed in the conventional method. The resulting fluorescent derivatives were separated by reversed-phase liquid chromatography on an ODS column by using a mixture of acetonitrile and 250mM acetate buffer (pH 4.6) and detected spectrofluorimetrically at 465nm with excitation at 350nm. The detection limit (signal-to-noise ratio=3) for Nacetylserotonin was 16 fmol per 20μl injection.
社団法人日本分析化学会の論文
2005-12-05