An Ultramicro Fluorimetric Determination of Total Ascorbic Acid in Human Serum Using 1,2-Diamino-4,5-dimethoxybenzene
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概要
- 論文の詳細を見る
A simple and sensitive fluorimetric method is described for the assay of total ascorbic acid, the sum of ascorbic acid and dehydroascorbic acid. in human serum. This is based on the reaction of dehydroascorbic acid with 1,2-diamino-4,5-dimethoxybenzene to give an intensely fluorescent product ; ascorbic acid is oxidized with iodine to dehydroascorbic acid beforehand. The method requires as little as 4μl of serum and the detection limit of ascorbic acid is 5.1 ng (28.3 pmol).
- 社団法人日本薬学会の論文
- 1985-08-25
著者
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大倉 洋甫
Faculty Of Pharmaceutical Sciences Kyushu University
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中村 優
福岡大学薬学部薬品分析学教室
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中村 優
Faculty Of Pharmaceutical Sciences Fukuoka University
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原 周司
Faculty Of Pharmaceutical Science Fukuoka University
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山口 政俊
Faculty Of Pharmaceutical Science Fukuoka University
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大倉 洋甫
Faculty Of Pharmaceutical Science Kyushu University
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大倉 陽甫
Faculty Of Pharmaceutical Sciences Kyushu University Katakasu Fukuoka
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