Rapid Enzyme Immunoassay for Human Choriogonadotropin in Serum using Horseradish Peroxidase as a Label
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概要
- 論文の詳細を見る
A rapid sandwich-type enzyme immunoassay of human choriogonadotropin in 20 μl of serum is described. Anti-human choriogonadotropin β subunitantibody (IgG fraction) was coated on an acrylonitrile-butadiene-styrene copolymer bead. The bead was incubated with choriogonadotropin in serum at 30℃ for 2 (or 3) h, and again incubated at 30℃ for 2 (or 3) h with antibody-labeled horseradish peroxidase prepared by Nakane and Kawaoi's method, to form a sandwich-type immunocomplex. The enzyme activity of the complex on the bead was measured by the use of a sensitive fluorogenic substrate, 3-(p-hydroxyphenyl) propionic acid. The method permits one-day assay of 0.0125-64 ng (2.75-14080 mIU) of the hormone.
- 社団法人日本薬学会の論文
- 1982-11-25
著者
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大倉 洋甫
Faculty Of Pharmaceutical Sciences Kyushu University
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中島 憲一郎
長崎大学大学院医歯薬学総合研究科
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財津 潔
Faculty of Pharmaceutical Sciences, Kyushu University 62
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財津 潔
Faculty Of Pharmaceutical Sciences Kyushu University 62
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中島 憲一郎
Faculty of Pharmaceutical Sciences, Nagasaki University
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秋山 修三
Faculty of Pharmaceutical Sciences, Nagasaki University
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秋山 修三
Faculty Of Pharmaceutical Sciences Nagasaki University
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秋山 修三
長崎大学薬学部
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大倉 洋甫
Faculty Of Pharmaceutical Science Kyushu University
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秋山 修三
Department Of Applied Physics And Chemistry Fukui University Of Technology
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