Preparation of [3-(2-Phyridyldithio)propionoyl]insulins and Horeseradish Peroxidase-Insulin Conjugates by High-Performance Liquid Chromatographic Separation
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概要
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Five [3-(2-pyridyldithio)propionoyl]insulins (PDP-insulins) were separated preparaticely from the reaction mixture of porcine insulin with N-succinimidyl 3-(2-phyidyldithio)propionate by means of anion-exchange high-preformance liquid chromatography on a TSK gel DEAE-SW column. Horseradich peroxidase (HRP)-insulin conjugates (Gly^<Al>-HRP-insulin, Lys^<B29>-HRP-insulin and Gly^<Al>, Lys^<B29>-diHRP-insulin) were prepared by the reaction of thiolated HRP and the corresponding PDP-insulins and purified by gel-permeation high-performance liquid chromatography on a TSK gel G3000SW column.
- 社団法人日本薬学会の論文
- 1988-04-25
著者
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大倉 洋甫
Faculty Of Pharmaceutical Sciences Kyushu University
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財津 潔
Faculty of Pharmaceutical Sciences, Kyushu University 62
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財津 潔
Faculty Of Pharmaceutical Sciences Kyushu University 62
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中山 正雄
Faculty of Pharmaceutical Sciences, Kyushu University
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中山 正雄
Faculty Of Pharmaceutical Sciences Kyushu University
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大倉 洋甫
Faculty Of Pharmaceutical Science Kyushu University
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大倉 陽甫
Faculty Of Pharmaceutical Sciences Kyushu University Katakasu Fukuoka
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