A Practical Reagent for Reversible Amino-Protection of Insulin, 3,4,5,6-Tetrahydrophthalic Anhydride
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概要
- 論文の詳細を見る
Of the examined dicarboxylic acid anhydrides, 3,4,5,6-tetrahydrophthalic anhydride was found to be the best reagent in practical use for reversible amino-protection of insulin in terms of the rapidity of acid-deprotection. Twelve Gly (A1), Phe (B1), Lys (B29)-triacyl-insulins were prepared by reaction of porcine insulin with the dicarboxylic acid anhydrides and time courses for the deprotection of the acylated insulins with dilute acetic acid were investigated by means of capillary zone electrophoresis, and the tetrahydrophthalyl (THP)-insulin obtained with the reagent was the most rapidly deacylated (6h, 25℃, 0.1M acetic acid). Isolation of triacyl-insulins and Gly (A1)-THP-, Gly (A1), Phe (B1)-diTHP- and Gly (A1), Lys (B29)-diTHP-insulins using DEAE anion-exchange high-performance liquid chromatography is also described.
- 社団法人日本薬学会の論文
- 1994-07-15
著者
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大倉 洋甫
Faculty Of Pharmaceutical Sciences Kyushu University
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財津 潔
Faculty of Pharmaceutical Sciences, Kyushu University 62
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財津 潔
Faculty Of Pharmaceutical Sciences Kyushu University 62
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木村 佳正
Faculty of Pharmaceutical sciences, Kyushu University 62
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本村 有史
Faculty of Pharmaceutical sciences, Kyushu University 62
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本村 有史
Faculty Of Pharmaceutical Sciences Kyushu University 62
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大倉 洋甫
Faculty Of Pharmaceutical Science Kyushu University
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大倉 陽甫
Faculty Of Pharmaceutical Sciences Kyushu University Katakasu Fukuoka
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木村 佳正
Faculty Of Pharmaceutical Sciences Kyushu University 62
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