Sensitive Fluorimetric Assay for Human Thyroid Peroxidase Using p-Cresol as a Substrate
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概要
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A sensitive fluorimetric method using p-cresol as a hydrogen-donating substrate is described for th assay of thyroid peroxidase in a crude preparation of human thyroid tissue. The fluorescence of the product formed enzymatically from p-cresol is measured at 410 nm with excitation at 325 nm in an alkaline solution (pH 12.3). The specific activities of thyroid peroxidase are 2 to 5 times higher in tissues from patients with Graves' disease than in normal tissues from patients with adenoma of the thyroid. This method is simple and sensitive enough to allow the enzyme assay to be carried out with as little as 10 mg of thyroid tissue.
- 公益社団法人日本薬学会の論文
- 1984-10-25
著者
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大倉 洋甫
(現)(株)同仁化学研究所
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甲斐 雅亮
九州大学薬学部
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甲斐 雅亮
Faculty of Pharmaceutical Sciences, Kyushu University 62
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野口 志郎
Noguchi Thyroid Clinical And Hospital Foundation
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大倉 洋甫
Faculty Of Pharmaceutical Science Kyushu University
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馬場 英俊
Noguchi Thyroid Clinical And Hospital Foundation
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