A Sensitive Enzymatic Method for the Fluorimetric Assay of Cholesterol in Serum and High-Density Lipoprotein
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概要
- 論文の詳細を見る
A highly sensitive fluorimetric method is described for the enzymatic assay of free and total cholesterol in serum and of cholesterol in serum high-density lipoprotein. Cholesterol is oxidized by means of the cholesterol oxidase-mediated reaction to give hydrogen peroxide, which is measured by a fluorimetric technique using the peroxidase-tyramine system. Cholesterol ester is hydrolyzed by means of the cholesterol ester hydrolase-catalyzed reaction. High-density lipoprotein is separated by the dextran sulfate-Mg^<2+> method. The method is simple, with good precision, and is extremely sensitive, requiring less than 1μl of serum for free or total cholesterol assay and less than 5μl of serum for high-density lipoprotein cholesterol assay.
- 公益社団法人日本薬学会の論文
- 1980-10-25
著者
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大倉 洋甫
Faculty Of Pharmaceutical Sciences Kyushu University
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財津 潔
Faculty Of Pharmaceutical Sciences Kyushu University 62
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濱田 千鶴子
Daiichi College of Pharmaceutical Sciences
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岩崎 正武
Daiichi College of Pharmaceutical Sciences
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大倉 洋甫
Faculty Of Pharmaceutical Science Kyushu University
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実渕 豊
Japan Medical Laboratory-Kyushu
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