Preparation of Acetylmercapto-3-carboxypropanol Insulins Using Preparative High-Performance Liquid Chromatography on an Anion-Exchange Column
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概要
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A method for the preparation of insulin derivatives which have protected sulfhydryl group(s) at definite site(s) on the molecule is described. Porcine insulin reacts with S-acetylmercaptosuccinic anhydride to afford four species of insulin derivatives that have 2 (or 3)-acetylmercapto-3-carboxypropanoyl group(s) at i) Gly(A1), ii) Gly(A1) and Phe(B1), iii) Gly(A1) and Lys(B29), and iv) Gly(A1), Phe(B1) and Lys(B29) positions. The derivatives are efficiently separated in a preparative scale by anion-exchange high-performance liquid chromatography on a TSKgel DEAE-2SW column. The four derivatives are all readily deacetylated with hydroxylamine to give the corresponding sulfhydryl insulin derivatives.
- 社団法人日本薬学会の論文
- 1992-07-25
著者
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大倉 洋甫
Faculty Of Pharmaceutical Sciences Kyushu University
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財津 潔
Faculty of Pharmaceutical Sciences, Kyushu University 62
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財津 潔
Faculty Of Pharmaceutical Sciences Kyushu University 62
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名波 雅彦
Faculty of Pharmaceutical Sciences, Kyushu University
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大倉 洋甫
Faculty Of Pharmaceutical Science Kyushu University
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大倉 陽甫
Faculty Of Pharmaceutical Sciences Kyushu University Katakasu Fukuoka
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名波 雅彦
Faculty Of Pharmaceutical Sciences Kyushu University
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