1,2-Diamino-4,5-methylenedioxybenzene as a Highly Sensitive Fluorogenic Reagent for α-Keto Acids(Analytical,Chemical)

概要

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The reactivity of pyruvic acid with 1,2-diaminobenzenes was investigated as part of a survey of fluorogenic reagents for a-keto acids of biological importance. Of eight 1,2-diaminobenzenes tested, 1,2-diamino-4,5-methylenedioxybenzene was found to be the best reagent in terms of sensitivity and reactivity. The reagent reacts with α-keto acids in an acidic solution to produce the corresponding fluorescent derivatives, which fluorescent most intensely in a neutral medium. The derivatives often a-keto acids can be separated within 18min by reversed-phase high-performance liquid chroma-tography with isocratic elution. The detection limits for the acids are at femtomole levels for an injection volume of 10μl. The fluorescent product from pyruvic acid was characterized as 3-methyl-6,7-methylenedioxy-2(1H)-quinoxalinone. The fluorescence properties of the product are also described.
社団法人日本薬学会の論文
1987-02-25