ジアセチルモノオキシム-グルクロノラクトン試薬による血液中の尿素窒素の微量定量(有機分析第63報)

概要

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A micromethod for determination of urea nitrogen in blood with diacetyl monoximeglucuronolactone reagent was proposed using 0.02ml. of sample without using any special equipment. Simplicity of the procedure and stability of the color produced permit analysis of a large number of samples at one time. The outline of the procedure is as follows. To 0.58ml. of water in a centrifuge tube, 0.020ml. of blood or serum is add and mixed, 0.20ml. of sodium tungstate solution (5%) is then added and mixed. To this mixture 0.20ml. of alminum potassium sulfate solution (4.8%) is added, mixed well, and centrifuged. From this solution, 0.50ml. of the supernatant clear solution is pipetted into a test tube, 0.50ml. of diacetyl monoxime-glucuronolactone solution (1% each in 5% acetic acid) and 3.0ml. of phosphoric acid solution (550ml./L.) are added successively, and mixed. The tube is headed in a boiling water bath for 40 minutes, cooled in running water, and the absorbance is read at 475mμ against a reagent blank.
公益社団法人日本薬学会の論文
1966-08-25