Effect of Spacer Groups Connecting Insulin with Fluorophore in Gly(A1)-sulfobenzoxadiazole-Labeled Insulins on the Immunoreactivity toward Anti-insulin Antibody
スポンサーリンク
概要
- 論文の詳細を見る
The immunoreactivity (specific binding) of three Gly(A1)-sulfobenzoxadiazole-labeled insulins which have different spacer groups, Gly(A1)-[3-(4-sulfobenzoxadiazol-7-ylthio)propanoyl]-insulin, Gly(A1)-[2-(4-sulfobenzoxadiazol-7-ylthio)acetyl]-insulin, Gly(A1)-[3-carboxy-2(or 3)-(4-sulfobenzoxadiazol-7-ylthio)propanoyl]-insulin, toward an antiporcine insulin monoclonal antibody was investigated, where solid-phase fluoroimmunoassay technique was utilized. The immunoreactivities of three labeled insulins were 2.1,1.8 and 1 in that order.
- 社団法人日本薬学会の論文
- 1993-05-15
著者
-
大倉 洋甫
Faculty Of Pharmaceutical Sciences Kyushu University
-
財津 潔
Faculty of Pharmaceutical Sciences, Kyushu University 62
-
財津 潔
Faculty Of Pharmaceutical Sciences Kyushu University 62
-
名波 雅彦
Faculty of Pharmaceutical Sciences, Kyushu University
-
大倉 洋甫
Faculty Of Pharmaceutical Science Kyushu University
-
大倉 陽甫
Faculty Of Pharmaceutical Sciences Kyushu University Katakasu Fukuoka
-
名波 雅彦
Faculty Of Pharmaceutical Sciences Kyushu University
関連論文
- Water-Soluble Carrier Proteins Having Carboxyl Spacer Groups
- High-Performance Liquid Chromatographic Separation of Citraconylinsulins and Preparation of Gly^Phe^-Dicitraconylinsulin
- Heterobifunctional Reagents for Cross-Linking of Sugar with Protein
- Determination of L-3,4-Dihydroxyphenylalanine in Plasma and Urine by High-Performance Liquid Chromatography with Fluorescence Detection(Analytical,Chemical)
- Fluorimetric Assay for Catechol-O-methyltransferase
- Fluorimetric Assay for Dopamine β-Hydroxylase in Rat Plasma
- A Sensitive Fluorometric Assay of Human Platelet Monoamine Oxidase and Its Application to Assessment of Monoamine Oxidase Inhibitor
- A Novel Fluorometric Assay Method of Dopamine-β-hydroxylase Activity in Human Serum
- HPLC-蛍光検出による人尿中イミダゾール酢酸及びN^τ-及びN^π-メチルイミダゾール酢酸の同時定量(発表論文抄録(1987年))
- Pre-column Fluorescence Derivatization High-Performance Liquid Chromatography of Opioid Peptides in Rat Brain and Its Use for Enzymatic Peptide Characterization
- A Practical Reagent for Reversible Amino-Protection of Insulin, 3,4,5,6-Tetrahydrophthalic Anhydride
- Preparative and Analytical Separations by High-Performance Liquid Chromatography and Capillary Electrophoresis of the Products from the Reaction of Insulin with exo-3,6-Epoxy-1,2,3,6-tetrahydrophthalic Anhydride
- Effect of Spacer Groups Connecting Insulin with Fluorophore in Gly(A1)-sulfobenzoxadiazole-Labeled Insulins on the Immunoreactivity toward Anti-insulin Antibody
- Preparation of Fluorescence Labeled Insulins, Sulfobenzoxadiazolyl-insulins, for Fluorescence Immunoassay
- Evaluation of the Numbers of Functional Groups Introduced into Horseradish Peroxidase in Reactions with Four Heterobifunctional Reagents
- Preparation of Acetylmercapto-3-carboxypropanol Insulins Using Preparative High-Performance Liquid Chromatography on an Anion-Exchange Column
- Preparation of S-Acetylthioglycoloyl Insulins Based on Separation by Anion-Exchage High-Performance Liquid Chromatography
- Solid-Phase Enzyme-Immunoassay of Anti-Insulin Antibiodies : Effect of Labeling Site in Insulin and of Labeled Number of Horseradish Peroxidase on the Assay Sensitivity
- Assay of Adenosine Deaminase in Serum by Flow-Injection Analysis with Fluorescence Detection
- Preparation of [3-(2-Phyridyldithio)propionoyl]insulins and Horeseradish Peroxidase-Insulin Conjugates by High-Performance Liquid Chromatographic Separation
- Assay of Purine Nucleoside Phosphorylase in Erythrocytes by Flow-Injection Analysis with Fluorescence Detection(Analytical,Chemical)
- New Heterobifunctional Cross-Linking Reagents for Protein Conjugation, N-(Bromoacetamido-n-alkanoyloxy)succinimides(Analytical,Chemical)
- Fluorimetric Assay for Hydrolytic Activity of Cholesterol Esterase in Rat Tissues
- p-スルファモイルベンジルアミン及びベンジルアミンを基質としたモノアミンオキシダーゼA及びBのけい光分析(発表論文抄録(1984年),福山大学開学10周年記念号)
- モノアミンオキシダーゼAおよびBの高速液体クロマトグラフィー : けい光検出による分析(発表論文抄録(1983年))
- New Chromogenic Substrates for the Assy of Esterases-Acetates and Butyrates of Phenolic Naphthylazo Compounds with Sulfonic Acid Group
- Rapid Enzyme Immunoassay for Human Choriogonadotropin in Serum using Horseradish Peroxidase as a Label
- Photo-decomposition of Captan and Difolatan
- 自動カラムスイッチング高速液体クロマトグラフィーによる血清中のアンピシリンの定量(品質管理・工程管理のための分析化学)
- A Fluorescent DNA Probe Prepared by the Direct Derivatization of the Sugar Moiety for Hydridization Assay
- Precolumn Derivatization of Nucleotides Based on Fluorescent Carbamate Formation on the Sugar Moieties in High-Performance Liquid Chromatogragphy
- Determination of 2', 3'-Dideoxyinosine and 2', 3'-Dideoxyadenosine in Rat Plasma by High-Performance Liquid Chromatography with Precolumn Fluorescence Derivatization
- 3-Bromomethyl-6,7-methylenedioxy-1-methyl-2(1H)-quinoxalinone as a Highly Sensitive Fluorescence Derivatization Reagent for Carboxylic Acids in High-Performance Liquid Chromatography
- 1,2-Diamino-4,5-methylenedioxybenzene as a Highly Sensitive Fluorogenic Reagent for α-Keto Acids(Analytical,Chemical)
- An Ultramicro Fluorimetric Determination of Total Ascorbic Acid in Human Serum Using 1,2-Diamino-4,5-dimethoxybenzene
- Fluorescent Products of Reaction between α-Keto Acids and 1,2-Diamino-4,5-dimethoxybenzene
- Fluorescent Product in the Determination of Aromatic Aldehydes with 4,5-Dimethoxy-1,2-diaminobenzene
- Mechanism of the Color Reaction between Aldehyde and Diazotized Aromatic Amine. II. Color Reaction Product of Benzaldehyde with Diazotized Sulfanilamide
- A Novel Time-Resolved Fluoroimmunoassay Using a Macrocyclic Europium Ligand as a Label
- A New Ligand for Europium(III) That Forms a Stable Fluorescent Complex in Aqueous Solution
- A New Photometric Method for the Determination of Serum Glutamate Pyruvate Transaminase Activity using Pyruvate and Glutamate as Substrates
- Spectrophotometric Assay for Lipase in Serum Using a Chromogenic Substrate, Orange I Laurate
- Fate of Hydralazine in Man. II. Formation of Tetrazolo [5,1-α] phthalazine in Vivo and Inhibition of Monoamine Oxidase by This Metabolite
- Fluorimetric Determination of Hexose with 5-Hydroxy-1-tetralone using Cupric Sulfate as an Accelerator of the Reaction
- Determination of Sulthiame in Plasma by High-Performance Liquid Chromatography
- 1,2-Diarylethylenediamines as Pre-column Fluorescence Derivatization Reagents in High-Performance Liquid Chromatographic Determination of Catecholamines in Urine and Plasma
- Simultaneous Determination of 2-Deoxy-D-glucose and D-Glucose in Rat Serum by High-Performance Liquid Chromatography with Post-Column Fluorescence Derivatization
- Fluorescence Reaction of Ribonucleosides and Ribonucleotides with 1,2-Bis(4-methoxyphenyl)ethylenediamine
- High-Performance Liquid Chromatographic Assay for Catechol-O-methyltransferase in Human and Rat Erythrocyte Membrane and Soluble Fractions, and Rat Tissues
- A New Photometric Assay Method of Serum Glutamate Dehydrogenase
- A New Colorimetric Method for the Determination of Serum Isocitric Dehydrogenase Activity
- Phosphorescence from 3,4-Dihydroxyphenyl Derivatives in Basic Solution and Its Use for the Assay of Epinephrine and Norepinephrine in Urine
- An Ultramicro Phosphorimetric Assay for Alkaline and Acid Phosphatase Activities
- Phosphorimetric Assay for 3-Methoxy-4-hydroxyphenylethyleneglycol in Urine
- Mechanism of the Color Reaction of Active Methylene Compounds with 1,3,5-Trinitrobenzene Derivatives. XI. The Jaffe Reaction
- Mechanism of the Color Reaction of Active Methylene Compounds with 1,3,5-Trinitrobenzene Derivatives. VIII. Interaction of Monoand Dihydroxylated Species (1 : 1 and 1 : 2 Complexes) derived from 1,3,5-Trinitrobenzene and Hydroxide Ion with Acid
- Reaction Products of 2-Oxoglutaric Acid with Diazotized Sulfanilamide
- A Sensitive Enzymatic Method for the Fluorimetric Assay of Cholesterol in Serum and High-Density Lipoprotein
- An Ultramicro Enzymatic Method for the Fluorimetric Determination of Uric Acid in Serum
- A New Fluorometric Assay for Human Serum Monoamine Oxidase
- Studies on Fluorometric Determination of Aromatic Aldehydes with 1,2 Diaminonaphthalene. II. On the Reaction Products of Benzaldehyde, Cinnamaldehyde, and Pyruvic Acid
- 醸造酢固形成分の血清コレステロール, 赤血球変形能に及ぼす効果
- Color Reaction Mechanism of Anthrone with Sugars. (2). Some Reaction Products of Furfural with Anthrone (Organic Analysis. XLVII)
- β-Diketone Derivative That Forms a Fluorescent Eu^ Complex in an Aqueous Solution
- Preparation and Evaluation of Fatty Acid Esters of 2-(4-Hydroxyphenyl)-4,5-diphenylimidazole as Fluorescent Substrates for Measurement of Lipase Activity
- Preparation and Evaluation of Fatty Acid Esters of Fluorescent p-Substituted Phenols as Substrates for Measurement of Lipase Activity(Analytical,Chemical)
- 自動カラムスイッチング高速液体クロマトグラフィーによる血しょう及び血清中セファレキシンの定量
- Determination of Nifedipine in Plasma by High-Performance Liquid Chromatography
- A Sensitive Fluorimetric Assay for Human Serum Monoamine Oxidase Activity
- Fluorimetric Assay of Histamine N-Methyltransferase by High-performance Liquid Chromatography
- Chemiluminescence Assay of N-Acetyl-β-D-glucosaminidase in Urine Using o-Aminophthalylhydrazido-N-acetyl-β-D-glucosaminide
- A New Chemiluminogenic Substrate for N-Acetyl-β-D-glucosaminidase, 4'-(6'-Diethylaminobenzofuranyl)phthalylhydrazido-N-acetyl-β-D-glucosaminide
- A Chemiluminogenic Substrate for N-Acetyl-β-D-glucosaminidase, o-Aminophthalylhydrazido-N-acetyl-β-D-glucosaminide
- New Water-soluble Hydrogen Donors for the Enzymatic Photometric Determination of Hydrogen Peroxide. II. N-Ethyl-N-(2-hydroxy-3-sulfopropyl) aniline Derivatives
- Mechanism of the Color Reaction between m-Dinitrobenzene and Alkali Cyanide. II. Color Reaction Products of 2,4-Dinitroaniline with Potassium Cyanide (Organic Analysis. LXXII)
- 醸造酢の人体の血中脂質, 血液レオロジーに及ぼす影響
- Sensitive Fluorimetric Assay for Human Thyroid Peroxidase Using p-Cresol as a Substrate
- テトラリン誘導体の研究(第2報)オキシテトラリン及びオキシテトラロンの紫外吸収スペクトル
- High-Performance Liquid Chromatographic Determination of Peptides Released by Tryptic Degradation from Opioid Peptide Precursors in Rat Brain
- 有機定性分析(第5報) : 芳香族炭化水素及びエーテルのベーパークロマトグラフィー
- Tetralin誘導体の研究(第1報) : 試験管内に於ける抗菌作用
- m-ジニトロベンゼン誘導体とシアン化アルカリの呈色反応機構(第3報) : 2,4-ジニトロフェノールとシアン化カリウムの反応生成物
- Mechanism of the Color Reaction between m-Dinitrobenzene Derivatives and Alkali Cyanide. (I). Some Reaction Products of m-Dinitrobenzene with Potassium Cyanide (Organic Analysis. LXVIII)
- イソチオシアネートの新比色定量法 : アプラナ科植物の研究, 第1報
- Mechanism of the Color Reaction of Pyruvic Acid with p-Dimethylaminobenzaldehyde. I. On the Reaction Products of Pyruvic Acid and p-Dimethylaminobenzaldehyde
- Colorimetric Determination of Pyruvic Acid with p-Dimethylaminobenzaldehyde
- Automated Determination of Urea Nitrogen in Serum with Diacetyl Monoxime-Glucuronolactone Reagent (Organic Analysis. LXXIII)
- ジアセチルモノオキシム-グルクロノラクトン試薬による血液中の尿素窒素の微量定量(有機分析 第63報)
- Organic Analysis. XXXV. Mechanism of the Color Reaction between Hexose and Thymol. (1).
- Organic Analysis. XIII. Estimation of Hexose with 5-Hydroxy-1-tetralone.
- Organic Analysis. X. Reaction Mechanism of 5-Hydroxytetralone with Glucose.
- 有機分析(第7報)5-オキシテトラロンによるヘキソーゼの新螢光反応
- 現代に生きる日本の伝統技術(2)醸造(つく)る--機能性食品としての"純米黒酢"
- 機器分析法の基礎知識--蛍光・りん光法
- 生体の超微量物質を追う--HPLC-蛍光検出法
- Mechanism of the Color Reaction of Active Methylene Compounds with 1,3,5-Trinitrobenzene Derivatives. VII. Color Reactions of Acetophenone with 1,3,5-Trinitrobenzene in Methanol and Methanol-Water Mixtures
- Mechanism of the Color Reaction of Active Methylene Compounds with 1,3,5-Trinitrobenzene Derivatives. VI. A Protonated Species of the Meisenheimer Type Compound derived from Picric Acid and Acetone
- Mechanism of the Color Reaction of Active Methylene Compounds with 1,3,5-Trinitrobenzene Derivatives. V. A New Type and the Meisenheimer Type Coloring Matters of the Janovsky Reaction of Acetophenone and 1,3,5-Trinitrobenzene
- Mechanism of the Color Reaction of Active Methylene Compounds with 1,3,5-Trinitrobenzene Derivatives. IV. Color Reaction of Picric Acid and Acetone under the Janovsky Conditions
- Determination of 3-Hydroxybutyric Acid in Blood via Acetone with Trinitrobenzene (Organic Analysis. LX)