Simultaneous Determination of 2-Deoxy-D-glucose and D-Glucose in Rat Serum by High-Performance Liquid Chromatography with Post-Column Fluorescence Derivatization
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概要
- 論文の詳細を見る
A simple high-performance liquid chromatographic method for the determination of 2-deoxy-D-glucose and D-glucose in rat serum is described; this method is based on a post-column fluorescence derivatization. The sugars are automatically converted into fluorescent derivatives by reaction with meso-1,2-bis(4-methoxyphenyl)ethylenediamine in an alkaline medium after their separation on a strong anion exchanger column (TSK gel Sugar AXG). The detection limits (S/N=3) for 2-deoxy-D-glucose and D-glucose in rat serum are 0.52 and 0.56 nmol/ml, respectively.
- 公益社団法人日本薬学会の論文
- 1990-04-25
著者
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大倉 洋甫
Faculty Of Pharmaceutical Sciences Kyushu University
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能田 均
Faculty of Pharmaceutical Sciences, Kyushu University
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能田 均
九州大学薬学部
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大倉 洋甫
Faculty Of Pharmaceutical Science Kyushu University
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大倉 陽甫
Faculty Of Pharmaceutical Sciences Kyushu University Katakasu Fukuoka
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梅香家 佳彦
Faculty of Pharmaceutical Sciences, Kyushu University
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梅香家 佳彦
Faculty Of Pharmaceutical Sciences Kyushu University
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