A Fluorescent DNA Probe Prepared by the Direct Derivatization of the Sugar Moiety for Hydridization Assay
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概要
- 論文の詳細を見る
The preparation of a fluorescent DNA probe based on the derivatization of the terminal hydroxyl group of the sugar moiety of a DNA primer and its applicability to the DNA hybridization assay are described. M13mp8 plasmid primer reacts with 2-(5-chlorocarbonyl-2-oxazolyl)-5,6-methylenedioxybenzofuran in the presence of sodium azide to form the corresponding fluorescent probe, which can be used for the hybridization assay to the target DNA, M13mp8 plasmid vector. The detection limit of the DNA with the naked eye is 10 ng (approximately 300 fmol)/spot on filters for the hybridization assay.
- 社団法人日本薬学会の論文
- 1992-10-25
著者
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大倉 洋甫
Faculty Of Pharmaceutical Sciences Kyushu University
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能田 均
Faculty of Pharmaceutical Sciences, Kyushu University
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能田 均
九州大学薬学部
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長岡 寛明
Faculty Of Pharmaceutical Sciences Kyushu University
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斉藤 幹彦
Dojindo Laboratories
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大倉 洋甫
Faculty Of Pharmaceutical Science Kyushu University
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大倉 陽甫
Faculty Of Pharmaceutical Sciences Kyushu University Katakasu Fukuoka
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