A New Chemiluminogenic Substrate for N-Acetyl-β-D-glucosaminidase, 4'-(6'-Diethylaminobenzofuranyl)phthalylhydrazido-N-acetyl-β-D-glucosaminide
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概要
- 論文の詳細を見る
A new type of chemiluminogenic substrate for N-acetyl-β-D-glucosaminidase was synthesized. The substrate was obtained by incorporation of an enzyme-removable N-acetyl-D-glucosaminide group to the cyclic hydrazide moiety of an arylic hydrazide which contains a highly fluorescent benzofuran framework. Following enzyme-mediated hydrolysis, the substrate released the hydrazide that was subsequently subjected to chemiluminescent oxidation with hydrogen peroxide and a hemin catalyst in an alkaline buffer to generate light. The detection limit of the enzyme using this substrate was 0.6 I.U./I.
- 公益社団法人日本薬学会の論文
- 1991-02-25
著者
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大倉 洋甫
Faculty Of Pharmaceutical Sciences Kyushu University
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佐々本 一美
Dojindo Laboratories
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大倉 洋甫
Faculty Of Pharmaceutical Science Kyushu University
-
大倉 洋甫
Faculty of Pharmaceutical Sciences, Kyushu University
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