Solid-Phase Enzyme-Immunoassay of Anti-Insulin Antibiodies : Effect of Labeling Site in Insulin and of Labeled Number of Horseradish Peroxidase on the Assay Sensitivity
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概要
- 論文の詳細を見る
Three horseradish peroxidase (HRP)-labeled porcine insulins which have definite labeling site(s) were compared regarding sensitivity in a solid-phase enzyme-immunoassay (EIA) of anti-insulin antibodies. The standard curves obtained with Lys^<B29>-HRP-insulin and Gly^<A1>-HRP-insulin were steeper than that with Gly^<A1>, Lys^<B29>-diHRP-insulin for both polyclonal and monoclonal antibodies. Thus, the mono-HRP-labeled insulins can afford higher sensitivities in the EIA. The importance of the HRP-labeling site in insulin and of the number of labeled HRP was first demonstrated by using HRP-labeled insulins having definite labeling site(s).
- 社団法人日本薬学会の論文
- 1991-02-25
著者
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大倉 洋甫
Faculty Of Pharmaceutical Sciences Kyushu University
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財津 潔
Faculty of Pharmaceutical Sciences, Kyushu University 62
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財津 潔
Faculty Of Pharmaceutical Sciences Kyushu University 62
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名波 雅彦
Faculty of Pharmaceutical Sciences, Kyushu University
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中山 正雄
Faculty of Pharmaceutical Sciences, Kyushu University
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中山 正雄
Faculty Of Pharmaceutical Sciences Kyushu University
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大倉 洋甫
Faculty Of Pharmaceutical Science Kyushu University
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大倉 陽甫
Faculty Of Pharmaceutical Sciences Kyushu University Katakasu Fukuoka
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名波 雅彦
Faculty Of Pharmaceutical Sciences Kyushu University
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