Fluorimetric Assay for Catechol-O-methyltransferase
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概要
- 論文の詳細を見る
A sensitive fluorimetric method for the assay of catechol-O-methyltransferase in a sample solution prepared from rat liver is described. Vanillin formed from the substrate, 3,4-dihydroxybenzaldehyde, under the optimal conditions for the enzyme reaction, is determined after the removal of unreacted substrate by adsorption on alumina. Vanillin is measured by the established fluorimetric method for the determination of aromatic aldehydes with 2,2'-dithiobis (1-aminonaphthalene). The limit of determination for vanillin formed is 300 pmol. The method is readily performed with good precision and is suitable for the rapid assay of catechol-O-methyltransferase in a small amount of sample solution.
- 社団法人日本薬学会の論文
- 1981-06-25
著者
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大倉 洋甫
Faculty Of Pharmaceutical Sciences Kyushu University
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財津 潔
Faculty of Pharmaceutical Sciences, Kyushu University 62
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大坪 健司
Hospital Pharmacy Faculty Of Medicine Kyushu University
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能田 均
Faculty of Pharmaceutical Sciences, Kyushu University
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能田 均
九州大学薬学部
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岡田 雄二
Faculty of Pharmaceutical Sciences, Kyushu University
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大坪 健司
Faculty of Pharmaceutical Sciences, Kyushu University
-
財津 潔
Faculty Of Pharmaceutical Sciences Kyushu University 62
-
大倉 洋甫
Faculty Of Pharmaceutical Science Kyushu University
-
岡田 雄二
Faculty Of Pharmaceutical Sciences Kyushu University
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