Evaluation of the Numbers of Functional Groups Introduced into Horseradish Peroxidase in Reactions with Four Heterobifunctional Reagents
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概要
- 論文の詳細を見る
Numbers of functional groups introduced into horseradish peroxidase in reactions with four heterobifunctional reagents, S-acetylmercaptosuccinic anhydride, N-(ε-maleimidocaproyloxy)succinimide, N-succinimidyl 3-(2-pyridyldithio)propionate and N-(3-bromoacetamido-n-propionoyloxy)succinimide, were investigated. In the reactions at large molar ratios of the reagents to the enzyme in neutral and slightly alkaline media, maximum numbers of the incorporated functional groups were approximately 2.2 : two reactive amino groups are present in the enzyme molecule.
- 社団法人日本薬学会の論文
- 1992-08-25
著者
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大倉 洋甫
Faculty Of Pharmaceutical Sciences Kyushu University
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財津 潔
Faculty of Pharmaceutical Sciences, Kyushu University 62
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財津 潔
Faculty Of Pharmaceutical Sciences Kyushu University 62
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名波 雅彦
Faculty of Pharmaceutical Sciences, Kyushu University
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大西 幹男
Faculty of Pharmaceutical Sciences, Kyushu University
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大西 幹男
Faculty Of Pharmaceutical Sciences Kyushu University
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大倉 洋甫
Faculty Of Pharmaceutical Science Kyushu University
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大倉 陽甫
Faculty Of Pharmaceutical Sciences Kyushu University Katakasu Fukuoka
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名波 雅彦
Faculty Of Pharmaceutical Sciences Kyushu University
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