プレカラム蛍光誘導体化を用いた高速液体クロマトグラフィーによる血清アンチパインの定量(<特集>医薬品の分析)

概要

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A high performance liquid chromatographic method is described for the determination of antipain which is a protease inhibitor and also a possible therapeutic drug for muscular destrophy. Antipain was converted to the fluorescent derivative by reaction with benzoin, a fluorogenic reagent for guanidino group. The derivative was then determined on a reversed phase column with isocratic elution within 15 min by using fluorescence detector. The detection limit of antipain in mouse serum was 0.42 μg/ml, which corresponds to the amount of 240 pg (400 fmol) in an injection volume of 100 μl at a signal to noise ratio of 2. The calibration graph for the compound was linear up to at least a concentration of 30 μg/ml in the serum. The proposed method was simple, rapid and sensitive enough to permit the quantification of antipain in as little as 40 μl of mouse serum dosed with the compound.
社団法人日本分析化学会の論文
1986-03-05