ジアゾ化p-クロロアニリンによる尿中フェニルピルビン酸の吸光光度定量

概要

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A sensitive and selective photometric method for the determination of phenylpyruvic acid in urine is described, based on the color reaction of the acid with diazotized ρ-chloroaniline in an alkaline medium. Phenylpyruvic acid in 0.1 ml of urine is extracted with ether at pH less than 1. The extract, after evaporating the solvent, is treated with diazotized ρ-chloro-aniline in the presence of sodium hydroxide, sodium sulfite and sodium hypophosphite at 37 ℃ for 30 min. The resulting formazan derivative is extracted with cyclohexane, and the absorbance of the extract is measured at 500 nm against the reagent blank. The amount of phenylpyruvic acid is calibrated by the standard addition method. This method permits the determination of the acid as low as 5 μg in 0.1 ml urine with good precision, and should be applicable for the acid in urine from patient with phenylketonuria.
社団法人日本分析化学会の論文
1981-07-05