生理活性物質の超微量分析 : 高選択的蛍光試薬開発からのアプローチ

概要

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Highly sensitive methods established in our laboratories are outlined for the determination of bioactive substances including oligopeptides and related enzyme activities (i.e. aromatic aldehydes, their precursors and related amine-metabolizing enzymes, α-keto acids, sialic acids, catecholamines, their metabolites and biosynthetic enzymes, biogenic guanidines, arginine- and tyrosine-containing peptides, reducing sugars, fatty acids involving prostaglandines, nucleosides and nucleic acid bases with active imino hydrogen and biogenic alcoholic compounds) in biological materials, by means of conventional fluorometry and mostly pre- and postcolumn fluorescence derivatization high performance liquid chromatography. A series of mono- and bifunctional fluorogenic reagents has been developed for the selective derivatization of such bioactive substances and as enzyme substrates. Their use in the methods is briefly referred in the text.
公益社団法人日本薬学会の論文
1988-01-25