発蛍光試薬1, 2-ジアミノ-4, 5-ジメトキシベンゼンを用いる食肉製品および魚肉ハム・ソーセージ中の亜硝酸塩の簡便・迅速なフローインジェクション分析

概要

論文の詳細を見る
A simple and rapid flow injection analysis(FIA)with fluorescence detection was developed for the determination of nitrite in meat and fish products(e.g., ham and sausages). 1, 2-Diamino-4, 5-dimethoxybenzene(DDB)was used as the fluorogenic reagent, and the carrier solution was 0.05 mM DDB in 0.5 M sulfuric acid which retained its availability for at least 48 h after preparation. The DDB derivative was monitored at 402 nm(ex.at 311nm). The calibration curve for standard nitrite ranging from 0.23 to 4.60μg / ml showed good linearity(r=0.998)between the concentration of nitrite and the fluorescence intensity. The limit for the detection of nitrite was 51 ng / ml at a signal-to-noise ratio of 3. The amount of nitrite in meat and fish products ranged from 7.8 to 24.1μg / g. Good correlation(r=0.991)was obtained between the proposed method and spectrophotometry by using a fish product spiked with a known concentration of standard nitrite.
社団法人日本家政学会の論文
2000-02-15