ピリミジン誘導体の分析化学的研究(第3報)6-アミノ-5-ニトロソ-4-オキソ-1-フェニル-2-チオキソ-1,2,3,4-テトラヒドロピリミジンを用いる鉄(II)の吸光光度定量

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Spectrophotometric determination of iron (II) with 6-amino-5-nitroso-4-oxo-1-phenyl-2-thioxo-1,2,3,4-tetrahydropyrimidine (ANTP) was investigated. ANTP reacts with iron (II) to form a water-soluble blue complex. Iron (II)-ANTP complex has an absorption maximum at 670 nm against the reagent blank and shows a definite absorbance over a range of pH 5.5 to 8.5. Beer's law holds over a range of 0-20 μg/10 ml of iron (II) at 670 nm, the molar absorption coefficient of the complex is 2.5×10^4 l・mol^<-1> cm^<-1>. Ni (II), Hg (II), Co (II), Pb (II), Bi (III), and citrate interfered with this determination. The molar ration of iron (II) to ANTP in the complex was 1 : 3. The established procedure is as follows : To a sample solution, 1 ml of 1% hydroxylamine hydrochloride solution, 2 ml of acetate buffer (pH 6), 2 ml of 0.01 M ANTP-dimethylformamide solution, and 1 ml of EtOH are added. The mixture is diluted to 10 ml with water, and its absorbance is measured at 670 nm against the reagent blank. This method was successfully applied to commercial tablets and capsule of iron (II).
公益社団法人日本薬学会の論文
1978-08-25