Development and Application of Organic Reagents for Analysis. V. High-Performance Liquid Chromatographic Determination of Lipoperoxides in Biological Fluids with 1,3-Diphenyl-2-thiobarbituric Acid

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A rapid, sensitive, and selective high-performance liquid chromatographic method has been developed for the quantification of lipoperoxides (malondialdehyde, MDA) in biological fluids (rat plasma and human serum) with visible detection of 1,3-diphenyl-2-thiobarbituric acid (DPTBA) condensate. The assay was linear up to 500 pmol per assay tube of MDA. The lower limit of detection of MDA was 10pmol per tube (S/N>2). and the coefficient of variation at the 50pmol/tube level was 2.6% (n=5). A linear relationship was obtained between the peak height and the amount of rat plasma or human serum (10-100μl). A comparison of the DPTBA-high-performance liquid chromatographic method with the thiobarbituric acid-colorimetric one was made for normal human sera. The correlation coefficient (r) was 0.954 with 18 samples. The method is simple and useful for routine assay of trace amounts of lipoperoxides in body fluids.
公益社団法人日本薬学会の論文
1985-12-25