A LAMINAR FLOW ABSORPTION MODEL FOR A CARRIER-MEDIATED TRANSPORT IN THE INTESTINAL TRACT

概要

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A laminar flow absorption model for a carrier-mediated transport was developed to analyze the data of the intestinal single perfusion experiment. By using this model, we calculated the absorption rate and the concentration of the substance (drug) at the aqueous-intestinal membrane interface (c_<wall>). The absorption rate was smaller than that predicted by the Michaelis Menten equation, using the concentration at the inlet (c_0). The apparent Michaelis constant (K_<m, app>) was larger than the true Michaelis constant (K_m), if the data were analyzed by using the simulation curve of the absorption rate vs. c_0 as the usual method. This is because c_<wall> was smaller than c_0. But the maximal transport velocity (V_<max>) was little affected, because C_<wall> became almost equal to c_0 when c_0 was high. By using this model, we can determine the values of V_<max> and K_m which are not biased by the unstirred water layer in the intestinal tract.
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