Correlation between Antioxidative Activities and Metabolite Changes during Cheonggukjang Fermentation
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概要
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Liquid chromatography mass spectrometry and multivariate analysis were employed to investigate the correlation between fermentation time-dependent metabolite changes in cheonggukjang, a traditional fermented soybean product, and changes in its antioxidant activity over 72 h. The metabolite patterns were clearly distinguished not by strains but by fermentation time, into patterns I (0–12 h), II (12–24 h), and III (24–72 h), which appeared as distinct clusters on principal component analysis. The compounds that significantly contributed to patterns I, II, and III were soyasaponins, isoflavonoid derivatives, and isoflavonoid aglycons respectively. Partial least square analysis for metabolite to antioxidant effects showed correlations between the ferric reducing/antioxidant power (FRAP) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay during 24–36 h, and 2,2′-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) test and total phenol content (TPC) during 36–72 h. Compared with the strong negative correlations of glucosylated-isoflavonoids with DPPH, ABTS and TPC during fermentation, the isoflavonoid aglycon displayed strong positive correlations with these compounds during fermentation.
- 2011-04-23
著者
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Lee Choong
Department Of Anatomy And Cell Biology College Of Veterinary Medicine And Bk21 Program For Veterinar
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KWON Dae
Korea Food Research Institute
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Kwon Dae
Department Of Biological Science And Engineering Korea Advanced Institute Of Science Of Technology
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KANG Daejung
Department of Bioscience and Biotechnology and Bio/Molecular Informatics Center, Konkuk University
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Kim Young
Department Of Food Science And Technology Ewha Womans University
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Choi Hyung-kyoon
College Of Pharmacy Chung-ang University
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Choi Jung
Departments Of Bioscience And Biotechnology Konkuk University
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Lee Choong
Department Of Bioscience And Biotechnology Konkuk University
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Kim Young-suk
Dep. Of Food Sci. And Technol. Ewha Womans Univ.
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Choi Hyung-kyoon
Coll. Of Pharmacy Chung-ang Univ.
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Kwon Dae
Department Of Bioscience And Biotechnology And Bio/molecular Informatics Center Konkuk University
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Kim Young-suk
Department Of Food Science And Technology Ewha Woman's University
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Kim Jiyoung
Department Of Bioscience And Biotechnology And Bio/molecular Informatics Center Konkuk University
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CHOI Jung
Molecular Informatics Center, Konkuk University
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SON Gun
Molecular Informatics Center, Konkuk University
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CHOI Hyung-Kyoon
Molecular Informatics Center, Konkuk University
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LEE Choong
Molecular Informatics Center, Konkuk University
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Son Gun
Department Of Bioscience And Biotechnology And Bio / Molecular Informatics Center Konkuk University
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Choi Jung
Department Of Agricultural Biotechnology Seoul National University
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KIM Young-Suk
Department of Food Science and Engineering, Ewha Womans University
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