Identification of Proteins that Regulate Radiation-induced Apoptosis in Murine Tumors with Wild Type p53
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概要
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In this study, we investigated the molecular factors determining the induction of apoptosis by radiation. Two murine tumors syngeneic to C3H/HeJ mice were used : an ovarian carcinoma OCa-I, and a hepatocarcinoma HCa-I. Both have wild type p53, but display distinctly different radiosensitivity in terms of specific growth delay (12.7d in OCa-I and 0.3d in HCa-I) and tumor cure dose 50% (52.6 Gy in OCa-I and>80 Gy in HCa-I). Eight-mm tumors on the thighs of mice were irradiated with 25 Gy and tumor samples were collected at regular time intervals after irradiation. The peak levels of apoptosis were 16.1±0.6% in OCa-I and 0.2±0.0% in HCa-I at 4h after radiation, and this time point was used for subsequent proteomics analysis. Protein spots were identified by peptide mass fingerprinting with a focus on those related to apoptosis. In OCa-I tumors, radiation increased the expression of cytochrome c oxidase and Bc12/adenovirus EIB-interacting 2 (Nip 2) protein higher than 3-fold. However in HCa-I, these two proteins showed no significant change. The results suggest that radiosensitivity in tumors with wild type p53 is regulated by a complex mechanism. Furthermore, these proteins could be molecular targets for a novel therapeutic strategy involving the regulation of radiosensitivity.
- 2007-09-15
著者
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Seong Jinsil
Departments of Radiation Oncology, Yonsei University Health System
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Kim Wonwoo
Departments of Radiation Oncology, Yonsei University Health System
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Oh Haejin
Department Of Radiation Oncology Brain Korea 21 Project For Medical Science Yonsei University Health
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Koom Woong
Department Of Radiation Oncology Brain Korea 21 Project For Medical Science Yonsei University Health
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Kim Wonwoo
Department Of Radiation Oncology Brain Korea 21 Project For Medical Science Yonsei University Health
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Kim Wonwoo
Department Of Radiation Oncology Brain Korea 21 Project For Medicine Yonsei University
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Kim Jiyoung
Department Of Radiation Oncology : Brain Korea 21 Project For Medical Science Yonsei University Medi
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Kim Jiyoung
Department Of New Materials Engineering Kookmin University
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Seong Jinsil
Department Of Radiation Oncology Brain Korea 21 Project For Medical Science Yonsei University Health
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Seong Jinsil
Department Of Radiation Oncology Brain Korea 21 Project For Medicine Yonsei University
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Seong Jinsil
Department Of Radiation Oncology : Brain Korea 21 Project For Medical Science : Institute For Gastro
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OH Hae
Departments of Radiation Oncology, Yonsei University Health System
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An Jeung
Department Of Radiation Oncology Brain Korea 21 Project For Medicine Yonsei University
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Oh Hae
Department Of Radiation Oncology Brain Korea 21 Project For Medicine Yonsei University
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Kim Jiyoung
Department Of Bioscience And Biotechnology And Bio/molecular Informatics Center Konkuk University
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Oh Hae
Department Of Radiation Oncology Brain Korea 21 Project For Medical Science Yonsei University Colleg
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Kim Wonwoo
Department Of Radiation Oncology Brain Korea 21 Project For Medical Science Yonsei University Colleg
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