A New Colorimetric Method for Determining the Isomerization Activity of Sucrose Isomerase

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概要

• 論文の詳細を見る

• A new colorimetric method for determining the isomerization activity of sucrose isomerase was developed. This colorimetric method is based on the enzymatic reactions of invertase and glucose oxidase-peroxidase (GOD-POD). The main scheme for assaying sucrose isomerase activity is to degrade sucrose in the reaction mixture to glucose and fructose by invertase and to detect the concentration of glucose generated using GOD-POD. The concentrations of trehalulose and isomaltulose, reaction products of sucrose isomerase, are calculated from the concentration of glucose. This method allows rapid and accurate determination of the isomerization activity of sucrose isomerase without inhibition by hydrolysis activity.

• 社団法人 日本農芸化学会の論文
• 2007-02-23

著者

• LIM Jin

  Department of Dental Biomaterials Science and Dental Research Institute, School of Dentistry, Seoul

• KIM Jeong

  Department of Information Electronics Engineering, Ewha Womans University

• KWON Dae

  Korea Food Research Institute

• KIM Jong-Sang

  Department of Animal Science and Biotechnology, Kyungpook National University

• Kim Jeong

  Department Of Anatomy Wonkwang University School Of Medicine

• KANG Daejung

  Department of Bioscience and Biotechnology and Bio/Molecular Informatics Center, Konkuk University

• Park Cheon-seok

  Institute Of Life Science And Resources Graduate School Of Biotechnology Kyunghee University

• Kim Jong-sang

  Department Of Animal Science And Biotechnology Kyungpook National University

• Lim Jin

  Department Of Animal Science And Biotechnology Kyungpook National University

• Park Sang-eun

  Institute Of Life Science And Resources Graduate School Of Biotechnology Kyunghee University

• CHO Mee-Hyun

  Institute of Life Science and Resources, Graduate School of Biotechnology, KyungHee University

• Cho Mee-hyun

  Institute Of Life Science And Resources Graduate School Of Biotechnology Kyunghee University

• Kwon Dae

  Department Of Bioscience And Biotechnology And Bio/molecular Informatics Center Konkuk University

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