Verification of the Intermolecular Parallel β-Sheet in E22K-Aβ42 Aggregates by Solid-State NMR Using Rotational Resonance : Implications for the Supramolecular Arrangement of the Toxic Conformer of Aβ42
スポンサーリンク
概要
- 論文の詳細を見る
Formation of the intermolecular β-sheet is a key event in the aggregation of 42-residue amyloid-β (Aβ42). We have recently identified a physiological and toxic conformer, the turn positions of which are slightly different from each other, in the aggregates of E22K-Aβ42 (one of the mutants related to cerebral amyloid angiopathy). However, it remains unclear whether the intermolecular β-sheet in the E22K-Aβ42 aggregates is parallel or antiparallel. We prepared an equal mixture of E22K-Aβ42 aggregates labeled at Cα and those labeled at C=O with 13C, whose intermolecular 13C–13C distance was estimated by solid-state NMR using rotational resonance (R2). The intermolecular proximity of β-strands at positions 21 and 30 was less than 6 Å, supporting the existence of the intermolecular parallel β-sheet in the E22K-Aβ42 aggregates as well as in wild-type Aβ42 aggregates. The results also suggest that each conformer would not accumulate alternately, but form a relatively large assembly.
- 社団法人 日本農芸化学会の論文
- 2008-08-23
著者
-
TAKEGOSHI Kiyonori
Department of Chemistry, Graduate School of Science, Kyoto University
-
Takegoshi Kiyonori
Graduate School Of Science Kyoto University
-
入江 一浩
京都大学農学部
-
入江 一浩
京都大学 食品工学
-
入江 一浩
京都大学 農
-
入江 一浩
京都大学大学院農学研究科食品生物科学
-
Shimosawa Tatsuo
Molecular Gerontology Tokyo Metropolitan Institute Of Gerontology
-
Irie Kazuhiro
京都大学 農学研究科
-
SHIMIZU Takahiko
Research Team for Molecular Biomarkers, Tokyo Metropolitan Istitute of Gerontology
-
SHIRASAWA Takuji
Research Team for Molecular Biomarkers, Tokyo Metropolitan Istitute of Gerontology
-
IRIE KAZUHIRO
Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University
-
MASUDA YUICHI
Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University
-
OHASHI RYUTARO
Department of Chemistry, Graduate School of Science, Kyoto University
-
Shimizu Takahiko
東京都老人総合研究所 老人バイオマーカー研究
-
Shimizu Takahiko
Research Team For Molecular Biomarkers Tokyo Metropolitan Institute Of Gerontology
-
Shirasawa Takuji
Research Team For Molecular Biomarkers Tokyo Metropolitan Istitute Of Gerontology
-
Shirasawa Takuji
Research Group Of Molecular Gerontology Tokyo Metropolitan Institute Of Gerontology
-
Irie Kazuhiro
Division Of Food Science And Biotechnology Graduate School Of Agriculture Kyoto University
-
Masuda Yuichi
Division Of Food Science And Biotechnology Graduate School Of Agriculture Kyoto University
-
Takegoshi Kiyonori
Department Of Chemistry Graduate School Of Science Kyoto University
-
NAKANISHI Azusa
Department of Chemistry, Graduate School of Science, Kyoto University
-
Ohashi Ryutaro
Department Of Chemistry Graduate School Of Science Kyoto University
-
入江 一浩
Division Of Food Science And Biotechnology Graduate School Of Agriculture Kyoto University
-
Nakanishi Azusa
Department Of Chemistry Graduate School Of Science Kyoto University
-
Shimosawa Tatsuo
Faculty Of Medicine The University Of Tokyo
関連論文
- Chemically Nonequivalent Sites in Mesoporous BCN Revealed by Solid-state NMR at 21.8T
- 1995年度日本農芸化学会大会【encircled 1】
- Antioxidant, EUK-8, Prevents Murine Dilated Cardiomyopathy
- 5 発がんプロモーター・インドラクタムの活性コンフォメーション(口頭発表の部)
- 発癌プロモーター・インドラクタムの活性コンフォメーション : 有機化学,天然物化学
- アミロイドβ(Aβ42)の毒性コンホメーションの提唱
- P-55 βアミロイドの新しい凝集モデルに基づく神経細胞毒性発現機構(ポスター発表の部)
- 11 固体NMRならびにESRによるβアミロイド(Aβ42)の毒性コンホメーションの解析(口頭発表の部)
- 1996年度日本農芸化学会大会 (1)
- 発癌プロモーター・テレオシジンの作用機構に関する有機化学的研究
- PE-433 Reduction in Hemoglobin-Oxygen Affinity Results in the Improvement of Exercise Capacity in Mice with Chronic Heart Failure(Heart failure, basic-4, The 71st Annual Scientific Meeting of the Japanese Circulation Society)
- 4(A-4) CH/π相互作用に着目したプロテインキナーゼCアイソザイム選択的な薬剤の分子設計(口頭発表の部)
- ペプチド合成によるホルボールエステル受容体の機能解析
- 連載講座 「もの」が語るバイオサイエンス(6)ペプチド合成によるホルボールエステル受容体の機能解析
- 113(P-62) 発がんプロモーター・インドラクタムVのコンホメーション固定によるプロテインキナーゼCアイソザイム選択的リガンドの分子設計(ポスター発表の部)
- プロテインキナーゼCのシステインリッチドメインの化学合成, 機能解析とドラッグデザイン
- 新規光反応性フォルポールエステルを用いたプロテインキナーゼC調節領域の光アフィニティーラベリング : 有機化学・天然物化学
- ポリフェノールによるアルツハイマー病予防の可能性
- βアミロイドの毒性コンホメーション
- Biological Significance of Isoaspartate and Its Repair System(D-Amino Acid Biosystem)
- 26 天然発がんプロモーターの骨格を利用した抗がん剤bryostatin等価体の開発(口頭発表の部)
- 'Malignant' Conformation of Alzheimer's β Peptides (Aβ42) through Radical Formation
- P-64 ELUICIDATION OF THE 'MALIGNATNT' CONFORMATION OF β-AMYLOID WITH ITALIAN MUTATION (E22K-Aβ42) USING SOLID-STATE NMR
- P-9 FORMATION AND STABILIZATION MECHANISM OF THE β-AMYLOID RADICAL
- Structure of β-Amyloid Fibrils and Its Relevance to Their Neurotoxicity : Implications for the Pathogenesis of Alzheimer's Disease
- Synthesis and Tumor-promoting Activities of 12-Epi-phorbol-12,13-dibutyrate
- Synthesis and Biological Activities of (-)-6-n-Octyl-indolactam-V, a New Potent Analogue of the Tumor Promoter (-)-Indolactam-V
- Synthesis and Biological Activities of Indolactone-V, the Lactone Analogue of the Tumor Promoter (-)-Indolactam-V
- Photocytotoxicity of Water-soluble Fullerene Derivatives
- Two Cases of Renovascular Hypertension and Ischemic Renal Dysfunction : Reliable Choice of Examinations and Treatments
- Adrenomedullin Amidation Enzyme Activities in Hypertensive Patients
- Measurement of Catechol-O-methyltransferase Activity in the Brain of Dahl Salt-Sensitive Rats(Analytical Biochemistry)
- Suppression of Catechol-O-Methyltransferase Activity through Blunting of α_2-Adrenoceptor Can Explain Hypertension in Dahl Salt-Sensitive Rats
- OE-330 Oxidative Stress Causes Heart Failure with Impaired Mitochondrial Respiration(Molecular biology, myocardium-1 (M) OE56,Oral Presentation (English),The 70th Anniversary Annual Scientific Meeting of the Japanese Circulation Society)
- 微生物転換を用いた6位置換インドラクタムの合成研究 : 有機化学,天然物化学
- 6 プロテインキナーゼCのホルボールエステル結合部位を含むサロゲートペプチドの合成と化学的特性(口頭発表の部)
- Analysis of ^B NMR Powder Lineshape of MgB_2 in the Normal Conductive Phase(Condensed matter: electronic structure and electrical, magnetic, and optical properties)
- フォルボールエステルのジアジリン誘導体の合成と特性 : 有機化学・天然物化学
- マウス皮膚PKC_ηの調節領域モデルペプチドの合成とその生化学的特性 : 有機化学・天然物化学
- P-38 ANALYSIS OF CH/π INTERACTION BETWEEN TUMOR PROMOTERS AND PROTEIN KINASE C C1 DOMAINS
- Binding Selectivity of Conformationally Restricted Analogues of (-)-Indolactam-V to the C1 Domains of Protein Kinase C Isozymes(Organic Chemistry)
- Selective Binding of Tumor Promoters to the Novel PKC C1B Domains
- Isolation of (-)-14-O-Malonylindolactam-V as a Possible Precursor of (-)-Indolactam-V and (-)-14-O-Acetylindolactam-V from Streptomyces blastmyceticum
- Tumor-Promoting Phorbol Esters Bind Directly to the Regulatory Domain of Diacylglycerol Kinase Gamma
- Synthesis and Characterization of Model Peptides Incorporating the Phorbol Ester-binding Domain of Protein Kinase C
- 発がんプロモーターのnPKC C1B ドメインへの選択的結合
- 3 Targeting Oxidative Stress in Preventing Cardiovascular Complications in Metabolic Syndrome(New Horizons in the Metabolic Syndrome:-Molecular Mechanisms and Therapies,Plenary Session 4 (PL4) (H),The 70th Anniversary Annual Scientific Meeting of the Japa
- Protective Effect of Adrenomedullin in Renal Interstitial Fibrosis and Relation to Lectin-Like Oxidized Low-Density Lipoprotein Receptor-1
- Organ-Protective Effects of Adrenomedullin
- Regional Hemodynamic Effects of Adrenomedullin in Wistar Rats: a Comparison with Calcitonin Gene-Related Peptide
- Adrenomedullin an endogenous antagonist against oxidative stress, Study with knock out mouse
- Caveolae are preferred sites for store-operated Ca^ linked to NO production
- Effect of aging on salt sensitivity of blood pressure in patients with essential hypertension
- Local Renin-Angiotensin System in Sympathetic Overactivity of Spontaneously Hypertensive Rats
- Standard organ weights among elderly Japanese who died in hospital, including 50 centenarians
- New Aggregation Model of Amyloid β by the Systematic Proline Replacement
- Synthesis and Phorbol Ester Binding of the Zinc-finger Like Sequences of All Protein Kinase C Isozymes
- OE-061 The Contributions of the Candidate Genes to Atherosclerosis are Not Uniform among the Arterial Systems(Atherosclerosis, basic-1 (H) OE11,Oral Presentation (English),The 70th Anniversary Annual Scientific Meeting of the Japanese Circulation Society)
- FRS-005 The Associations between the Genetic Polymorphisms of Pro- and Anti-inflammatory Chemokine/Cytokines and the Atherosclerotic Degrees in the Japanese Elderly(Frontier of Atherosclerosis Research (H) FRS1,Featured Research Session,The 70th Anniversa
- PJ-197 Left Ventricular Abnormal Relaxation is Induced by Reactive Oxygen Species in Dahl Salt Sensitive Rats(Heart failure, basic-6 (M) PJ33,Poster Session (Japanese),The 70th Anniversary Annual Scientific Meeting of the Japanese Circulation Society)
- Inhibition by Genistein of the Lipopolysaccharide-Induced Down-Regulation of Programmed Cell Death 4 in RAW 264.7 Mouse Macrophages
- Identification of Toxic Conformation in Aβ42 Aggregates Using Solid-state NMR
- Model mice for tissue-specific deletion of the manganese superoxide dismutase gene
- In Vitro Covalent Binding Proteins of Zerumbone, a Chemopreventive Food Factor
- In Vitro Covalent Binding Proteins of Zerumbone, a Chemopreventive Food Factor
- Inhibition by Genistein of the Lipopolysaccharide-Induced Down-Regulation of Programmed Cell Death 4 in RAW 264.7 Mouse Macrophages
- Silymarin Attenuated the Amyloid β Plaque Burden and Improved Behavioral Abnormalities in an Alzheimer's Disease Mouse Model
- The turn formation at positions 22 and 23 in the 42-mer amyloid β peptide : The emerging role in the pathogenesis of Alzheimer's disease
- Verification of the Intermolecular Parallel β-Sheet in E22K-Aβ42 Aggregates by Solid-State NMR Using Rotational Resonance : Implications for the Supramolecular Arrangement of the Toxic Conformer of Aβ42
- CH/π相互作用を利用した薬剤開発(最前線,ポストゲノム時代の有機化学)
- Silymarin Attenuated the Amyloid β Plaque Burden and Improved Behavioral Abnormalities in an Alzheimer’s Disease Mouse Model
- Synthesis and Biological Evaluation of the 12,12-Dimethyl Derivative of Aplog-1, an Anti-Proliferative Analog of Tumor-Promoting Aplysiatoxin
- Suppression of CD74 Expression and Helicobacter pylori Adhesion by Auraptene Targeting Serum Starvation-Activated ERK1/2 in NCI-N87 Gastric Carcinoma Cells
- プロテインキナーゼ C 機能ドメインの化学合成
- プロテインキナーゼCの2つのzinc fingerモチーフの機能と役割
- Teleocidin類の構造活性相関と蛍光標識化
- プロテインキナーゼCのホルボールエステル結合部位の光アフィニティーラベリングによる構造解析
- プロテインキナーゼCのホルボールエステル結合部位の合成と化学的特性
- Synthesis and Biological Evaluation of the 12,12-Dimethyl Derivative of Aplog-1, an Anti-Proliferative Analog of Tumor-Promoting Aplysiatoxin
- 33. 気孔形成を調節するペプチドホルモンの機能解析(口頭発表,植物化学調節学会第46回大会)
- プロテインキナーゼCのホルボールエステル結合部位の合成と化学的特性
- 1,2-Di-O-α-linolenoyl-3-O-β-galactosyl-sn-glycerol as a Superoxide Generation Inhibitor from Perilla frutescens var. crispa
- Stimulation of the Amyloidogenic Pathway by Cytoplasmic Superoxide Radicals in an Alzheimer's Disease Mouse Model
- Identification and Biological Activities of Bryostatins from Japanese Bryozoan
- 11B Nuclear Magnetic Resonance Study on Existence of Boron–Hydrogen Complex in Boron-Doped Diamond
- Molecular Dynamics of Octyl Urea Crystals Analyzed by Solid-state NMR