3-ハイドロキシ-2-ピロンのアミノ酸アナライザーによる分析

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When 3-hydroxy-2-pyrone (HP) was allowed to react with ninhydrin, a yellow coloration occurred. The molar absorption coefficient of HP in the ninhydrin reaction was 6.12 ×103 at 440nm, whereas that of L-proline was 5.64 ×103. When HP was analyzed by an amino acid analyzer (Hitachi KLA-5), the peak of HP (pK 1 1.62) appeared ahead of the peak of L-aspartic acid (pK1 1.87). HP was found to be separated on the cation exchange resin and could be determined by the amino acid analyzer from the relation between HW value of the peak and mmol amount of HP. The recovery of HP from the column was 99%. When dehydroascorbic acid (DHA) was heated in aqueous solution (pH 2) in a boiling water bath and the solution was analyzed by the amino acid analyzer, the peaks of 2, 3-diketogulonic acid, DHA and HP with one unidentified peak were obtained. By heating for 30min, 0.53mmol of HP was formed from 1mmol of DHA.
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