マウス肝部分切除後の肝再生に対するインターフェロンの抑制作用に関する生化学的研究

概要

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The effects of interferon (IFN) on DNA synthesis, cAMP levels, and induction of ornithine decarboxylase activity (ODC) were examined in the regenerating mouse liver after partial hepatectomy. IFN administration suppresed [3H] thymidine incorporation into DNA to 30% of that of the control mice after patial hepatectomy. To find why IFN suppresed DNA synthesis in the regenerating liver, we tested the effects of IFN on the intracellular levels of cAMP and polyamines, which were both important in cell growth. IFN inhibited adenylate cyclase activity, resulting in the suppresion of cAMP levels. IFN also suppressed the induction of ODC and elevation of polyamine levels. The inhibitions by IFN of ODC induction and DNA synthesis were reversed by db-cAMP and putrescine, respectively. These results suggest that IFN suppresses accumulation of cAMP and polyamine levels, and thus inhibits DNA synthesis in the regenerating liver.