Interaction between 7-Anilino-4-methylcoumarin-3-acetic Acid and Human Serum Albumin(Analytical,Chemical)
スポンサーリンク
概要
- 論文の詳細を見る
The fluorescence characteristics of 7-anilino-4-methylcoumarin-3-acetic acid (AMCA) were examined in human serum albumin (HSA) solution. The fluorescence of AMCA was significantly enhanced in the presence of HSA. Scatchard plots and continuous variation plots indicated that one molecule of AMCA binds to HSA. The binding parameters of AMCA were also estimated. Specific binding sites on HSA were examined in connection with Sudlow's classification. The primary binding site of AMCA was found to be site III (digitoxin site) from the results of competitive displacement studies with several drugs. Furthermore, it was suggested from the fluorescence behavior in the presence of tryptophan-, tyrosine-, and lysine-modified HSAs that the binding site of AMCA lies in a region near a tryptophan residue on HSA. The present data indiate that AMCA is useful as a site III marker.
- 社団法人日本薬学会の論文
- 1987-05-25
著者
-
入倉 充
Faculty Of Pharmaceutical Sciences
-
合屋 周次郎
Pharmaceutical Institute, Medical Faculty, University of Kyushu
-
合屋 周次郎
熊本大学薬学部
-
高舘 明
第一薬大
-
合屋 周次郎
Pharmaceutical Institute Medical Faculty University Of Kyushu
-
高舘 明
Pharmaceutical Sciences, Kumamoto University
-
入倉 充
Pharmaceutical Sciences, Kumamoto University
-
OHKUBO YOSHIKO
Pharmaceutical Sciences, Kumamoto University
-
小田切 優樹
Pharmaceutical Sciences, Kumamoto University
-
Ohuchi Yukie
Department Of Radiopharmacy Tohoku Pharmaceutical University
-
大窪 芳子
Department Of Radiopharmacy Tohoku Pharmaceutical University
-
大窪 芳子
熊本大学 薬
関連論文
- アルコール性水酸基の螢光ラベル化剤としての2-Dansylethyl Chloroformateの合成と性質
- Characterization of Drug Binding Sites on α_1-Acid Glycoprotein
- Characterization of Binding Sites for Sulfadimethoxine and Its Major Metaboite, N^4-Acetylsulfadimethoxine, on Human and Rabbit Serum Albumin
- Organic Analysis. XVI. Ultraviolet Spectral Study of Substituent Effect on Benzenesulfonamide
- The Modified High-Density Survival Assay is the Useful Tool to Predict the Effectiveness of Fractionated Radiation Exposure
- Relationship between Liver Injury and Transglutaminase Activities in Guinea Pigs and Rats(Pharmacology)
- 6位および7位置換3-Aminoisocarbostyril誘導体の合成(第2報)3-Alkylamino-および3-Arylamino-isocarbostyril誘導体の合成
- 6位および7位置換3-Aminoisocarbostyril誘導体の合成(第1報)Methyl(2-Cyanomethyl)benzoate誘導体とアミン類との反応
- カルボン酸けい光ラベル化剤としてのN, N'-Dicyclohexyl-O-(7-methoxycoumarin-4-yl)methylisoureaの合成および性質
- 分析試薬としてのクマリン誘導体の合成およびそのけい光特性
- 2-Amino-3-hydrazinoisocarbostyril誘導体のs-Triazolo[1,5-b]isoquinolin-5(1H)-oneおよび1,2-Dihydro-6H-s-tetrazino[2,3-b]isoquinolin-6-oneへの閉環反応
- 2,3-Diaminoisocarbostyril誘導体の1H, 5H-s-Triazolo[1,5-b]isoquinolin-5-oneおよび6H-as-Triazino[2,3-b]isoquinolin-6-oneへの閉環反応
- 3-Aminoisocarbostyril誘導体の合成およびその一級アミンとの反応
- フロセミドの体内動態に及ぼす尿毒症物質3-carboxy-4-methyl-5-propyl-2-furanpropanoic acidの影響
- The Effects of Several Vasopressin Receptor Antagonists on Normal Intraocular Pressure and the Intraocular Distribution of Vasopressin Receptor Subtypes
- ヒト血清アルブミン分子上の薬物結合サイトのミクロ環境解析:サイトI内のサブサイトIcについて
- Characterization of Region Ic in Site I on Human Serum Albumin. Microenvironmental Analysis Using Fluorescence Spectroscopy
- Study on the Binding of Dicumarol to α_1-Acid Glycoprotein Using Circular Dichroism Spectroscopy
- Studies on Metabolism of 3-Desoxyestrone. I. Synthesis of 16,17-Oxygenated Estra-1,3,5 (10)-trienes
- 7-Alkylaminocoumarin-4-acetic Acids as Fluorescent Probe for Studies of Drug-Binding Sites on Human Serum Albumin
- 3-(7-Methoxycoumarin-3-carbonyl)- and 3-(7-Dimethylaminocoumarin-3-carbonyl)-2-oxazolones as New Fluorescent Labeling Reagents for High-Performance Liquid Chromatography
- Interaction between 7-Anilino-4-methylcoumarin-3-acetic Acid and Human Serum Albumin(Analytical,Chemical)
- Interaction of the Fluorescent Probe 7-Anilino-4-methylcoumarin-3-acetic Acid with α-Globulin
- New Labeling Reagents for Alcohols in Fluorescence High-Performance Liquid Chromatography
- Interaction between Methacycline and Human Serum Albumin
- アゾジカルボン酸ジエチルおよびトリフェニルホスフィン存在下4-ヒドロキシメチル-7-メトキシクマリンによるカルボン酸類のけい光ラベル化反応
- Epimerization of C-17 Substituent during Formation of Isobufadienolide
- 3-(7'-Methoxycoumarin-3'-carbonyl)-benzoxazoline-2-thioneによるアマンタジンの蛍光ラベル化及び高速液体クロマトグラフィー
- Pyrene-1-carbonyl Fluorideによるアルコール類及びアミン類の蛍光ラベル
- アミン類の蛍光誘導体化試薬7-Dimethylaminocoumarin-3-carbonyl Fluorideの合成及びその反応性
- アルコール類の蛍光誘導体化試薬Pyrene-1-carbonyl Azideの合成及びその反応性
- アルコール類の蛍光誘導体化試薬7-Dimethylaminocoumarin-3-isocyanateの合成及びその反応性
- A Fluorogenic Reagent : 3-(4,6-Difluorotriazinyl)amino-7-methoxycoumarin, for the Determination of Amantadine by High-Performance Liquid Chromatography
- アルコール類の蛍光誘導体化試薬Anthracene-1-carbonyl Azideの合成及びその反応性
- 3-(Difluoro-1,3,5-triazinyl)-1-(ethylthio)-2-n-propylbenz[f]isoindole as a Fluorescence Derivatization Reagent for Estrogens in High-Performance Liquid Chromatography
- 蛍光誘導体化試薬1-(Difluoro-1,3,5-triazinyl)-2-methylisoindoleの合成及びコルチコステロイド類との反応性
- 二次蛍光検出高速液体クロマトグラフィーによるp-ベンゾキノン類の定量
- 新規蛍光誘導体化試薬1-(Dichloro-1,3,5-triazinyl)-2-methylisoindoleの合成及びエストロゲン類との反応性(第2報) : 妊婦尿中エストリオールの定量
- 新規蛍光誘導体化試薬1-(Dichloro-1,3,5-triazinyl)-2-methylisoindoleの合成及びエストロゲン類との反応性
- Fluorogenic Reaction of Sulfite with o-Phthalaldehyde
- 5-Dimethylaminonaphthalene-1-sulfonyl Azide によるオレフィン類の蛍光ラベル化反応
- A Convenient Derivatization with Anion Exchange Resin Catalysts for High-Performance Liquid Chromatographic Analysis. I. Derivatization of Estrogens with Dansyl Chloride
- 果たして情報化社会だろうか(情報化時代を迎えた薬学図書館のあり方)
- The Effect of Blood Decrease on ^Ga Uptake by the Liver in Partially Hepatectomized Rats(Biopharmacy)
- Mechanism of Inhibition of Small Intestinal Motility by Restraint Stress Differs from That with Norepinephrine Treatment in Rats
- Activation of μ-Opioid Pathway Is Associated with the Canceling Effect of Footshock Stimulus on the Restraint Stress-Induced Inhibition of Small Intestinal Motility in Rats
- β_3-Adrenoceptor Is Involved in the Inhibition of Small Intestinal Motility Due to Restraint Stress in Rats
- Evaluation of the Effects of Restraint and Footshock Stress on Small Intestinal Motility by an Improved Method Using a Radionuclide, ^51Cr, in the Rat
- Possible Neural Mechanisms Involved in Footshock Stress-Induced Enhancement of Exploratory Behavior in Mice
- 3-Aminoisocarbostyril誘導体の蛍光特性 その3 イソカルボスチリル及びその類縁化合物の蛍光特性
- 3-Aminoisocarbostyril誘導体の螢光特性 その2 2-Amino-3-hydrazino-および2,3-Diamino-isocarbostyrilのヒドラゾンおよび環化成績体の発光特性
- 3-Aminoisocarbostyril誘導体の螢光特性 その1
- Electronic Properties of N-Heteroaromatics. XLVII. Absorption Spetra and Electronic Structure of Some Quinoline 1-Oxides of Biological Interest
- Clinically relevant radioresistant cells efficiently repair DNA double-strand breaks induced by X-rays
- Gene Expression Profiles in Mouse Liver Cells after Exposure to Different Types of Radiation
- Transferrin Is Not Involved in the Entry of^Ga into Hepatocytes from Regenerating Liver of Partially Hepatectomized Rats
- N-異項還化合物のSm-MeSO_3H系による還元反応
- β-シクロデキストリン包接による7位置換クマリン誘導体の螢光挙動
- Synthesis and Properties of 4-Diazomethyl-7-methoxycoumarin as a New Fluorescent Labeling Reagent for Alcohols and Carboxylic Acids
- The Uptake Mechanism of Gallium-67 into Hepatocytes Treated with Carbon Tetrachloride(Biochemistry)
- The Involvement of Transferrin in the Uptake of Iron-59 by Hepatocytes of Carbon Tetrachloride-Damaged Rats(Pharmacology)
- The Involvement of Polyamines as Substrates of Transglutaminase in Zonal Different Hepatocyte Proliferation after Partial Hepatectomy(Biochemistry/Molecular Biology)
- Uptake of Gallium-67 by the Hepatocytes during Liver Regeneration in Carbon Tetrachloride-Treated Mice(Biochemistry/Molecular Biology)
- Zonal Differences in DNA Synthesis and in Transglutaminase Activity between Perivenous versus Periportal Regions of Regenerating Rat Liver(Biochemistry/Molecular Biology)
- カルボン酸けい光ラベル化剤としてのダンシル誘導体の合成および反応
- Oxidative Stress Induced Lipocalin 2 Gene Expression : Addressing its Expression under the Harmful Conditions
- Characterization of Binding Site of Uremic Toxins on Human Serum Albumin
- New Fluorescence Probes for Drug-Albumin Intereaction Studies
- Free ^Ga enters into the hepatocytes of carbon tetrachloride-treated rats
- Development of an Experimental System for Evaluation of Stress Effect on Ethyl Alcohol Metabolism Using Radiorespirometric Analysis in Rat (Pharmacology)
- Zonal differences in gallium-67 uptakes between perivenous versus periportal regions of rat liver following carbon tetrachloride treatment
- Studies on Steroid Conjugates. VI. Synthesis of 17α-Estradiol 17-N-Acetylglucosaminide
- Synthesis of 16-Substituted 14,17-cis-5α-Cardenolides
- Synthesis of 14,15-Epoxyandrostan-17β-yl-isoxazoles and -pyrazoles
- Synthesis of 14,15-Epoxyisobufadienolides
- Conformational Analysis of Epimeric 16-Bromo-13α-estrones
- Synthesis of 16-Methyl-14,17-cis-5-dehydro- and -5α-Isobufadienolides
- Reactions of 14β, 15β-Epoxypregn-16-en-20-one with Nucleophiles
- The Synthesis and Conformational Analysis of Epimeric 16-Bromo-5α, 13α-androstan-17-ones
- A Nuclear Magnetic Resonance Spectral Study of Some 5α, 14β-Androstanes
- 薬物タンパク結合の研究における蛍光分光法の利用
- The Entering of Indium-111 and Iron-59 into the Hepatocytes from Partially Hepatectomized Rats Differ from That of Gallium-67(Biochemistry/Molecular Biology)
- Effect of Restraint and Footshock Stress and Norepinephrine Treatment on Gastric Emptying in Rats
- Studies on Tetralin Derivatives. XI. Synthesis of 5-Hydroxy-6-acyl-1,4-naphthoquinones
- Organic Analysis. XVII. Some Observations on Ultraviolet Spectra of Phenyl Sulfone Derivatives
- Studies on Tetralin Derivatives. X. Some Derivatives of 2,3-Dihydroxytetralin
- テトラリン誘導体の研究(第2報)オキシテトラリン及びオキシテトラロンの紫外吸収スペクトル
- N, N'-Dicyclohexyl-およびN, N'-Diisopropyl-O-(7-methoxycoumarin-4-yl)methylisoureaによるケトカルボン酸のけい光ラベル化反応
- ヘテロ環化合物の研究(第3報) : 4,5-Diamino-6-substituted-aminopyrimidine類のプリン誘導体への閉環反応