Fluorogenic Reaction of Sulfite with o-Phthalaldehyde

概要

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o-Phthalaldehyde (OPA) was found to react with sodium sulfite in borate buffer (pH 9.9) to form a fluorescent product (λ_<ex> 360nm, λ_<em> 424nm), which was stable only in the solution. The fluorescence of the product in alkaline solution was stable for at least 6h. Hydrosulfite and pyrosulfite showed the same degree of fluorescence intensity as sulfite. Other sulfur compounds gave little or no fluorescence. The working curve for sodium sulfite was linear in the range of 0.2-7.0nmol/2ml. The detection limit of sodium sulfite was 0.2nmol/2ml. The presence of metal ions had practically no influence on the fluorescence intensity of the OPA-sodium sulfite system. However, contamination with ammonium ion and amines should be avoided in the determination of sulfite. The fluorescent product seems to be formed through a mechanism different from that of isoindole formation, but its structure has not yet been determined.
公益社団法人日本薬学会の論文
1986-03-25