The Modified High-Density Survival Assay is the Useful Tool to Predict the Effectiveness of Fractionated Radiation Exposure
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概要
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The high-density survival (HDS) assay was originally elaborated to assess cancer cell responses to therapeutic agents under the influence of intercellular communication. Here, we simplified the original HDS assay and studied its applicability for the detection of cellular radioresistance. We have recently defined clinically relevant radioresistant (CRR) cells, which continue to proliferate with daily exposure to 2 gray (Gy) of X-rays for more than 30 days in vitro. We established human CRR cell lines, HepG2-8960-R from HepG2, and SAS-R1 and -R2 from SAS, respectively. In an attempt to apply the HDS assay to detect radioresistance with clinical relevance, we simplified the original HDS assay by scoring the total number of surviving cells after exposure to X-rays. The modified HDS assay successfully detected radioresistance with clinical relevance. The modified HDS assay detected CRR phenotype, which is not always detectable by clonogenic assay. Therefore, we believe that the modified HDS assay presented in this study is a powerful tool to predict the effectiveness of fractionated radiotherapy against malignant tumors.
- 2010-05-16
著者
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KUWAHARA Yoshikazu
Department of Pathology, Institute of Development, Aging and Cancer, Tohoku University
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FUKUMOTO Manabu
Department of Pathology, Institute of Development, Aging and Cancer, Tohoku University
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SHIMURA Tsutomu
Institute of Industrial Science, University of Tokyo
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MORI Miyuki
Department of Pathology, Institute of Development, Aging and Cancer, Tohoku University
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OIKAWA Toshiyuki
Department of Pathology, Institute of Development, Aging and Cancer, Tohoku University
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SHIMURA Tsutomu
Department of Pathology, Institute of Development, Aging and Cancer, Tohoku University
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OHTAKE Yosuke
Department of Radiopharmacology, Tohoku Pharmaceutical University
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MORI Shiro
Division of Oral and Maxillofacial Surgery, Department of Oro-Maxillofacial Surgical Science, Tohoku
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OHKUBO Yasuhito
Department of Radiopharmacology, Tohoku Pharmaceutical University
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Fukumoto Manabu
Department Of Pathology Institute Of Development Aging And Cancer Tohoku University
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Oikawa Toshiyuki
Department Of Pathology Institute Of Development Aging And Cancer Tohoku University
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Shimura Tsutomu
Kyoto University Radiation Biology Center
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Shimura Tsutomu
京都大学放射線生物研究センター
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Shimura Tsutomu
Department Of Pathology Institute Of Development Aging And Cancer Tohoku University
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Kuwahara Yoshikazu
Department Of Pathology Institute Of Development Aging And Cancer Tohoku University
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Ohtake Yosuke
Dep. Of Radiopharmacology Tohoku Pharmaceutical Univ.
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Fukuoka Masako
岡山大学 薬学部 衛生代謝解析学
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Shimura Tsutomu
Radiation Biology Center Kyoto Univ.
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Mori Miyuki
Department Of Pathology Institute Of Development Aging And Cancer Tohoku University
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Mori Shiro
Division Of Oral And Maxillofacial Surgery Department Of Oro-maxillofacial Surgical Science Tohoku U
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Fukumoto Manabu
Dept. Pathol. Idac. Tohoku Univ.
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Fukumoto M
First Department Of Pathology School Of Medicine Kyoto University
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Ohuchi Yukie
Department Of Radiopharmacy Tohoku Pharmaceutical University
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Ohkubo Yasuhito
Department Of Radiopharmacology Tohoku Pharmaceutical University
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Fukumoto Manabu
Department Of Pathology And Biology Of Disease Faculty Of Medicine Kyoto University
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大窪 芳子
Department Of Radiopharmacy Tohoku Pharmaceutical University
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Ohtake Yosuke
Department Of Radiopharmacology Tohoku Pharmaceutical University
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Shimura Tsutomu
Radiat. Biol. Center. Kyoto. Univ.
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MORI Miyuki
Department of Animal and Marine Bioresource Sciences, Graduate School of Agriculture, Kyushu University, Fukuoka 812-8581, Japan
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