桃仁の研究(第3報)桃仁の抗炎症性蛋白成分PR-Bの抗活性酸素作用

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PR-B is a protein composition of Persicae semen (mol. wt.=about 10000) ; and its antiinflammatory activity is comparable to that of indomethacin in the carrageenin-induced hind paw edema test. In order to explore the mode of anti-inflammatory action of PR-B, inhibitory activities of PR-B on kinin-release by kallikrein and superoxide anion production were investigated in vitro. PR-B produced a dose-related inhibition of kinin-release by kallikrein at concentrations of 10^<-5>-10^<-3>M, and its potency (IC_<50>=1.4×10^<-4>M) was comparable to that of soybean trypsin inhibitor (SBTI) ; superoxide dismutase (SOD) and N-α-p-tosyl-L-lysine-chrolomethylketone, a synthetic trypsin inhibitor were inactive. The potency of PR-B as a trypsin inhibitor (IC_<50>=3.2×10^<-4> M or more than 10^<4-> M) was much weaker than that of SBTI (IC_<50>=7.2 or 7.4×10^<-8>M) ; SOD was inactive. In contrast to those activities, PR-B had comparatively strong SOD-like activity ; i.e., PR-B inhibited dose-relatedly the superoxide anion production by peritoneal macrophages of guinea pigs at concentrations of 10^<-6>-5×10^<-5>M, and its potency (IC_<50>=5.7×10^<-6>M) was approximately equivalent to that of SBTI while much weaker than that of SOD (IC_<50>=5.7×10^<-9>M). Lysozyme and egg albumin as reference proteins lacked the SOD-like activity. The anti-edema activity of PR-B given intravenously was stronger than that of SBTI and SOD in the carrageenininduced hind paw edema test in rats. These results were discussed in connection with the mode of anti-inflammatory action of PR-B.
社団法人日本薬学会の論文
1985-09-25

桃仁の研究(第3報)桃仁の抗炎症性蛋白成分PR-Bの抗活性酸素作用

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