Synthesis of Transferrin-Mitomycin C Conjugate as a Receptor-Mediated Drug Targeting System
スポンサーリンク
概要
- 論文の詳細を見る
Macromolecular conjugates of mitomycin C (MMC) were sunthesized by binding an active ester of glutarylated MMC (MMC-G-OSu) to human holo-transferrin (TF). Water-soluble TF-MMC conjugates (TF-G-MMC) were obtained in a good yield (>95%) by this method. The MMC content of the conjugate increased (0.82-9.49 MMC/w%) with increasing amounts of MMC-G-OSu added to the conjugation mixture. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis showed no aggregation in these conjugates. ^<125>I-TF-G-MMC was bound specifically to the TF receptor on Sarcoma 180 cells; the measurement of equilibrium binding of the ^<125>I-labeled conjugate resulted in a saturation isotherm. The amount of conjugate specifically bound to the TF receptor decreased as the MMC content of the conjugate increased. However, it was found that the conjugate with an MMC content below 10 mol MMC/mol TF still retains a binding activity of more than half that of TF. Therefore, when an optimal chemical modification was chosen, TF could be used as a tumor specific drug carrier.
- 公益社団法人日本薬学会の論文
- 1996-05-15
著者
-
Tanaka Tetsuro
Laboratory Of Biopharmaceutics Faculty Of Pharmacy And Pharmaceutical Sciences Fukuyama University
-
Tanaka T
Department Of Biopharmaceutics Faculty Of Pharmacy & Pharmaveutical Sciences Fukuyama University
-
Tanaka Tetsuro
Department Of Electronics Faculty Of Engineering Kyoto University
-
Tanaka Tetsuro
Department Of Electrical And Electronics Engineering Kagoshima University
-
Tanaka T
Institute For Health Care Science Suntory Ltd.
-
Tanaka T
Suntory Consumer Health Products Division
-
Miyashita Michiko
Faculty Of Pharmaceutical Science Hokuriku University
-
Tanaka T
School Of Pharmaceutical Sciences Nagasaki University
-
KANEO Yoshiharu
Department of Biopharmaceutics, Faculty of Pharmacy & Pharmaceutical Sciences, Fukuyama University
-
MIYASHITA Masahide
Department of Biopharmaceutics, School of Pharmacy, Fukuyama University
-
Kaneo Yoshiharu
Laboratory Of Biopharmaceutics Faculty Of Pharmacy And Pharmaceutical Sciences Fukuyama University
-
Kaneo Yoshiharu
Faculty Of Pharmacy & Pharmaceutical Sciences Fukuyama University
-
Kaneo Yoshiharu
Department Of Biopharmaceutics School Of Pharmacy Fukuyama University
-
Tanaka Tetsuro
Department Of Biopharmaceutics School Of Pharmacy Fukuyama University
関連論文
- Penicillium、FusariumまたRoselliniaにより発酵処理した緑茶エキスの化学成分
- 目で見る漢方薬・生薬(12)芍薬
- 植物ポリフェノールに関する化学的研究とその紅茶色素生成機構解明への展開
- ポリマー断片化によるプロアントシアニジンオリゴマーの製造方法
- P-21 紅茶色素形成に関わるエピガロカテキン由来ビシクロ[3.2.1]オクタン骨格を持つ鍵中間体の生成分解機構(ポスター発表の部)
- 茶葉とビワ葉の混合発酵茶に含まれる香気成分の特性
- 混合発酵茶の製造法と発酵におけるビワ葉の役割 (特集 健康機能性食品の開発)
- ナス(Solanum melongena L.)培養組織の成分解析
- 三番茶葉とビワ葉を混合揉捻した新たな発酵茶の開発
- 102(P-59) (±)-Scopadulinの全合成(ポスター発表の部)
- ウスバサイシンAsiasarum sieboldii F. Maekawaの発芽特性
- DPPH radical-scavenging effect on some constituents from the aerial parts of Lippia triphylla
- 7-O-Galloyl-D-sedoheptulose Is a Novel Therapeutic Agent against Oxidative Stress and Advanced Glycation Endproducts in the Diabetic Kidney(Pharmacognosy)
- Protective Effects of Morroniside Isolated from Corni Fructus against Renal Damage in Streptozotocin-Induced Diabetic Rats
- Identification of Antidiabetic Effect of Iridoid Glycosides and Low Molecular Weight Polyphenol Fractions of Corni Fructus, a Constituent of Hachimi-jio-gan, in Streptozotocin-Induced Diabetic Rats
- Two new maltol glycosides and cyanogenic glycosides from Elsholtzia rugulosa Hemsl.
- Rubusuaviins A-F, Monomeric and Oligomeric Ellagitannins from Chinese Sweet Tea and Their α-Amylase Inhibitory Activity
- Studies of Anti-cataract Drugs from Natural Sources. I. Effects of a Methanolic Extract and the Alkaloidal Components from Corydalis Tuber on in Vitro Aldose Reductase Activity
- Antihypertensive Effect of Sesamin. III. Protection against Development and Maintenance of Hypertension in Stroke-Prone Spontaneously Hypertensive Rats
- Ellagitannins and Lignan Glycosides from Balanophora japonica (Balanophoraceae)
- Caffeoyl, Coumaroyl, Galloyl, and Hexahydroxydiphenoyl Glucoses from Balanophora japonica
- Mechanisms of Gastric Mucus Secretion from Cultured Rat Gastric Epithelial Cells Induced by Carbachol, Cholecystokinin Octapeptide, Secretin, and Prostaglandin E_2
- Gastric Mucin Secretion from Cultured Rat Epithelial Cells
- Some Factors Which Influence Intrinsic Factor Content and Its mRNA Level in the Rat Stomach
- Regulation of Gastric Mucosal Pepsinogen and Intrinsic Factor Contents, and Their mRNA Levels during Starvation and Refeeding in Rats
- Inactivation of Blasticidin S by Bacillus cereus. VI. Structure and Comparison of the bsr Gene from a Blasticidin S-Resistant Bacillus cereus
- Nucleotide Sequence of the bsr Gene and N-Terminal Amino Acid Sequence of Blasticidin S Deaminase from Blasticidin S Resistant Eshcerichia coli TK121(Biological Chemistry)
- Cloning and Expression of a New Structural Gene for Blasticidin S Deaminase, a Nucleoside Aminohydrolase(Biological Chemistry)
- Biflavanones, Diterpenes, and Coumarins from the Roots of Stellera chamaejasme L.
- Activity-Guided Fractionation of Green Tea Extract with Antiproliferative Activity against Human Stomach Cancer Cells
- Revised Structure of Cercidinin A, a Novel Ellagitannin Having (R)-Hexahydroxydiphenoyl Esters at the 3, 4-Positions of Glucopyranose
- Arachidonic Acid-Induced Hind Limb gangrene : A New Experimental Rat Model of Paripheral Vascular Disease
- Interaction of Peptides Having a Turn Structure with Tannins
- Euscaphinin, a new ellagitannin dimer from Euscaphis japonica (THUNB.) KANITZ.
- Polymer-Like Polyphenols of Black Tea and Their Lipase and Amylase Inhibitory Activities
- Structures of Epicatechin Gallate Trimer and Tetramer Produced by Enzymatic Oxidation
- Characterization of the CYP2C8 Active Site by Homology Modeling
- Characterization of the CYP3A4 Active Site by Homology Modeling
- Effects of Iridoids on Lipoxygenase and Hyaluronidase Activities and Their Activation by β-Glucosidase in the Presence of Amino Acids
- Iridoids and Anthraquinones from the Malaysian Medicinal Plant, Saprosma scortechinii (Rubiaceae)
- Antiproliferative Constituents in the Plant 8. Seeds of Rhynchosia volubilis
- Studies on a Medicinal Parasitic Plant: Lignans from the Stems of Cynomorium songaricum
- Alkaloids, Diarylheptanoid and Naphthalene Carboxylic Acid Ester from Rhoiptelea chiliantha
- A Novel Rearranged Type of Secoeudesmane Sesquiterpenoide from the Root of Lindera strychinifolia (SIEB. et ZUCC.) F. VILLARS
- New Phenylpropanoid Glycosides from the Fruits of Illicium anisatum
- Dammarane-Type Triterpene Glycosides from the Leaves of Rhoiptelea chiliantha
- Non-Ohmic and Oscillatory Behaviors at Strong Electric Field in Tellurium
- Anti-Stress Effect of Oolong Tea in Women Loaded with Vigil
- Influence of Histamine in a Liver Injury Model Induced by Propionibacterium acnes and Lipopolysaccharide(Pharmacology)
- Optical Energy Gap of the Mixed Crystal CdS_xTe_
- Phase Diagram of the CdS-CdTe Pseudobinary System
- Uptake of Pullulan in Cultured Rat Liver parenchymal Cells(Biopharmacy)
- Dietary Supplementation of L-Carnosine Prevents Ischemia/Reperfusion-Induced Renal Injury in Rats(Pharmacology)
- Effects of Sesamin on Aortic Oxidative Stress and Endothelial Dysfunction in Deoxycorticosterone Acetate-Salt Hypertensive Rats (Pharmacology)
- Inhibitory Effect of Oolong Tea on the Oxidative State of Low Density Lipoprotein (LDL)
- Antihypertensive Effect of Sesamin. IV. Inhibition of Vascular Superoxide Production by Sesamin
- Preventive Effect of a Chicken Extract on the Development of Hypertension in Stroke-prone Spontaneously Hypertensive Rats(Food & Nutrition Science)
- Antihypertensive Effects of Chicken Extract against Deoxycorticosterone Acetate-Salt-Induced Hypertension in Rats
- Receptor Mediated Endocytosis and Cytotoxicity of Trandferrin-Mitomycin C Conjugate in the HepG2 Cell and Primary Cultured Rat Hepatocyte
- Intracellular Disposition and Cytotoxicity of Transferrin-Mitomycin C Conjugate in HL60 Cells as a Receptor-Mediated Drug Targeting System
- Polysaccharides as Drug Carriers : Biodisposition of Fluorescein-Labeled Dextrans in Mice
- Synthesis of Transferrin-Mitomycin C Conjugate as a Receptor-Mediated Drug Targeting System
- Synthesis and Evaluation of Water-Soluble Poly(vinyl alcohol)-paclitaxel Conjugate as a Macromolecular Prodrug(Biopharmacy)
- Synthesis of poly(vinyl alcohol)-doxorubicin conjugates containing cis-aconityl acid-cleavable bond and its isomer dependent doxorubicin release
- Pharmacokinetics and Biodisposition of Poly(vinyl alcohol) in Rats and Mice
- Anisotropic Piezoresistance of Cadmium Antimonide
- Further Metabolism of 4-Acetylaminoantipyrine, the Major Metabolite of Aminopyrine, in Rats(Pharmacological)
- BIOHYDROXYLATION OF AMINOPYRINE : QUANTITATIVE STUDIES OF 3-HYDROXYMETHYL METABOLITE IN RATS
- Effects of Oolong Tea on Metabolism of Plasma Fat in Mice under Restraint Stress(Food & Nutrition Science)
- Se Film Piezoelectric Transducer Deposited on Te Crystal
- Hypoglycemic Action of Cyclocarya paliurus (Batal.) Iljinskaja in Normal and Diabetic Mice(Food & Nutrition Science)
- Hypolipcmic Effect of Cyclocarya paliurus (Batal) Iljinskaja in Lipid-Loaded Mice
- Epitaxial Growth of Cd_xHg_Te
- Testing the Controllability of Discrete-Time Linear Systems with Input Constraints
- Positive Controllability Test of Discrete-Time Linear Systems
- Controllability of Multiple Input Discrete-Time Linear Systems with Positive Controls
- Blue-Emitting Diodes of 6H-SiC Prepared by Chemical Vapor Deposition
- Deposition and Properties of Polycrystalline Si for Solar Cells : I-1: SILICON SOLAR CELLS (I)
- Optical Properties of β-SiC Crystals Prepared by Chemical Vapor Deposition
- Piezoelectric Properties of Se Film Deposited on Te Crystal
- Evaluation of Child Care Practice Factors That Affect the Occurrence of Sudden Infant Death Syndrome : Interview Conducted by Public Health Nurses
- Derivation of Hydroxamic Acid from Pectin and Its Applications in Colorimetric Determination
- Galvanomagnetic Effects in Single Crystals of Cadmium Arsenide
- Preparation of a High Mobility Thin Film of Cd_3As_2
- The Electrooptic Effect in Cubic ZnSe at 10.6 μ
- Epitaxial Growth of CdTe by a Close-Spaced Technique
- Ultrasonic Amplification in Tellurium
- Current Oscillation in Tellurium at High Electric Field
- Temperature Dependence of Nonlinear V-I Characteristic in Tellurium
- Electrical Properties of Undoped p-CdSb at Low Temperatures
- Negative Magnetoresistance in Ag Doped p-CdSb
- New Blue Photoluminescence of Ga-Doped 4H-SiC Grown from Si Melt
- Photoluminescence of 4H-SiC Single Crystals Grown from Si Melt
- Photon Assisted Tunneling in SiC LED's Prepared by Overcompensation Method
- Defect Luminescence in SiC Light-Emitting Diodes
- Fundamental Properties of MIS Solar Cells : I-2: SILICON SOLAR CELLS (II)
- MIS Silicon Solar Cells with In_2O_3 Antireflective Coating
- Piezoelectric Effects in Selenium Rectifiers
- Current Oscillation in Dark-Conductive CdS
- Galvanomagnetic Effects in Cadmium Antimonide