精密ろ過ホローファイバー培養装置における培養条件のtPA生産に及ぼす影響

概要

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The effects of culture conditions on the production of tissue plasminogen activator (tPA) were studied with recombinant baby hamster kidney (BHK) cells in a micro-filtration hollow fiber bioreactor using Dulbecco's Modified Eagle medium with serum. The dissolved oxygen concentration (DO) and pH of the medium had a strong influence on tPA productivity, and it was optimal for tPA production to control the pH at 7.2 and the DO at an air saturated concentration. When tPA was harvested from the extra-capillary space (EC), the production was 1.25-fold that when it was harvested from the intra-capillary space (IC). tPA production was maintained at an almost constant level throughout the culture, even though the serum concentration was reduced from 5 to 0.2%. When the culture conditions were set at pH 7.2,DO 6.5 mg/l, serum concentration 0.2%, and EC harvesting, the mean tPA production was 335 mg/d, or 172 mg/d/m^2 of the hollow fiber membrane. Stable continuous cultivation was achieved for 78 d, and 20 g of tPA was produced in total after the serum concentration was reduced to 0.2%.
社団法人日本生物工学会の論文
1997-03-25