精密ろ過ホローファイバー培養装置を用いた組換えBHK細胞によるtPAの連続生産

概要

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Continuous cultivation of recombinant Baby Hamster Kidney (BHK) cells producing tissue plasminogen activator (tPA) was carried out in a micro-filtration hollow fiber (MFHF) bioreactor using Dulbecco's Modified Eagle's medium with serum. Stable, continuous cultivation for 66 d was possible, compared with a stoppage of cultivation at 40 d in the case of an ultra-filtration hollow fiber (UFHF) bioreactor. After the cells were fully grown, the serum concentration was decreased from 5% to 1%, but the tPA productivity remained at the same level continuously. The mean tPA concentration was 17.1 mg/l and the mean daily tPA production was 590 mg/d. The tPA productivity was 150 mg/d/m^2 in the case of the MFHF bioreactor. As the medium in the MFHF bioreactor was evenly supplied from the intra-capillary to the extra-capillary space, the tPA productivity of this bioreactor was 16% better than that of the UFHF type. The simplified culture system of the MFHF bioreactor make it suitable for commercial-scale tPA production.
社団法人日本生物工学会の論文
1996-11-25