膜型バイオリアクターを用いたphoプロモーターからの遺伝子発現の誘導と物質生産の効率化

概要

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Escherichia coli and Saccharomyces cerevisiae containing recombinant plamids in which the E. coli β-galactosidase gene was fused just downstream of phoA or PHO5 promoters were cultured to obtain large amounts of gene product efficiently. The gene expressions from these promoters were dependent on the inorganic phosphate concentration in the medium. With recombinant E. coli, gene expression was repressed above 1 mM of phosphate. In order to attain high productivity of the recombinant protein, we tried a two step-culture in which the cell growth phase was separated from the successive production phase. For removal of phosphate, we employed a membrane bioreactor. By the cross-flow filtration, phosphate could be completely removed and β-galactosidase of 114 μkat/l with E. coli and 210 μkat/l with S. cerevisiae was produced.
社団法人日本生物工学会の論文
1993-09-25