多孔性セルロース担体によるハイブリドーマ細胞の固定化とモノクローナル抗体の連続生産

概要

論文の詳細を見る
Macroporous cellulosic support modified by introducing polyethyleneimine was used for immobilization of animal cells. By introduction of a positive charge to the support, the number of cells attached increased. In particular, anchorage-independent hybridoma cells were immobilized more than 10-fold compared with the unmodified support. In the production of monoclonal antibody by perfusion culture using a fluidized-bed bioreactor, although a high cell density of more than 10^7 cells/ml could be obtained, the expected amount of antibody was not recovered in the spent medium. We found that free cells leaked from the support were killed by air bubbles, and that the cell debris aggregated and formed precipitates. The antibodies seemed to be adsorbed to the precipitates. To prevent cell death and precipitate formation by air bubbling, Pluronic F68 was added to the medium. Due to the protective effects of the reagent, leaked cells were not killed, and a large amount of antibody was continuously produced over a long term. The antibody productivity per unit reactor volume was improved approx. 20-fold compared with static cultures.
1993-05-25