動物細胞培養における有用物質の生産と分離に関する研究

概要

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High cell density culture is essential for the efficient production of biological substances such as monoclonal antibody and hormones by cultured animal cells, since animal cells usually have a low growth rate and produce small amount of product. Novel cell immobilization methods using photo-crosslinkable resins and macroporous cellulosic supports were developed to achieve high cell density. The hybridoma and recombinant L-929 cells immobilized were cultured in bioreactors and the productivities of monoclonal antibody and erythropoietin were improved very much. In hybridoma cell culture using serum-free medium, the effects of dissolved oxygen concentration and growth substrate concentrations such as glucose and glutamine were examined with respect to the metabolism and antibody production. Furthermore, the separation and concentration method of monoclonal antibodies by aqueous two-phase extraction using Pluronic F68 was proposed.
公益社団法人日本生物工学会の論文
1994-09-25