〓過型培養槽によるD-乳酸の連続生産

概要

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<I>Sporolactobacillus inulinus</I> was cultured in a bioreactor equipped with a hollow fiber module, and continuous production of D-lactate was examined. In batch culture, concentration of D-lactate produced was estimated on line with the aids of a pulse counter and a personal computer from the amount of alkaline solution used for neutralizing the medium. When this monitoring system was applied to the hollow fiber bioreactor, the estimated lactate concentration was slightly lower than the measured one. In this culture, ammonium ion produced by the microorganism disturbed the estimation.However, by reducing yeast extract concentration in the medium or by introducing a suitable correction factor in the equation for estimation, a fairly good estimation followed by the good control of the lactate concentration could be attained. Furthermore, the cell concentration was also monitored by a turbidimeter and controlled at a desired level by removing cells automatically from the fermentor. The specific growth rate and the lactate production rate were controlled at constant values by keeping both cells and lactate concentrations constant.
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