Bacillus licheniformis由来耐熱性α-アミラーゼを生産する麹菌の育種と液化仕込への利用

概要

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Aspergillus oryzae transformants which produced Bacillus licheniformis thermophilic α-amylase (optimum temperature, 76℃ ; optimum pH, 9) were bred using Aspergillus niger No.8AN promoter. When an A.oryzae transformant was grown on rice koji and wheat bran koji, 150g total rice was liquefied by koji representing 13% of the total rice and by extraction of 10g wheat bran koji, respectively. When small-scale brewing tests with liquefied rice and the transformant koji or extraction of wheat bran koji were carried out, the brewing profile and sake analysis values were similar to the results of tests carried out using a control enzyme (CP-40). The results indicate that A.niger No.8AN promoter can be applied to the production of heterologous proteins such as B.licheniformis thermophilic α-amylase in A.oryzae.
社団法人日本生物工学会の論文