Streptomyces rochei DB-34が生産する細胞壁溶解酵素による各種酵母のプロトプラスト形成と再生

概要

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The lytic enzyme produced by Streptomyces rochei DB-34 has a broad lytic spectrum. The enzyme lysed not only many ascomycetaseae and imperfecti yeasts in the logarithmic phase but also basidiomycetaseae yeasts regardless of the growth phase. However, protoplasts were not formed with this enzyme when sugars or sugar alcohols were used as an osmotic stabilizer; for their formation, osmotic stabilization by sodium tartrate was necessary. Sodium tartrate did not activate the enzyme, but seemed to enhance the sensitivity of the cell surface against it. This effect appeared only in the logarithmic phase of cell growth. Protoplasts were not regenerated on an agar medium coexisting with sodium tartrate. For the regeneration of protoplasts, sorbitol was effective as an osmotic stabilizer. In a system with sorbitol, protoplasts formed using DB-34 enzyme showed a higher regeneration efficiency than those formed using other commercially available lytic enzymes. The efficiency of transformation through the protoplasts was as good as that using Zymolyase 100T.
社団法人日本生物工学会の論文
1999-04-25