特異的モノクローナル抗体による火落菌の免疫化学的分類と抗体の認識する細胞表層抗原の解析

概要

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A total of 855 strains of hiochi bacteria were isolated from sake from four sake breweries, and the reactivity spectra of ten monoclonal antibodies (MAbs) specific to these isolates were examined by enzyme-linked immunosorbent assay (ELISA). It was found that spectral comparison could be used as a highly sensitive and rapid procedure for immunochemical classification of the hiochi bacteria, and that the distribution of hiochi bacteria varied considerably in the sake from one brewery to another. It is suggested that all the MAbs recognized hiochi bacterial cell-surface proteins. Of the ten MAbs, three(ho-H H7,ho-H D1 and he-H H34)recognized a portion of the carbohydrate of their protein. Furthermore, their antigen was present over all the cell surface.
1996-03-25