赤色酵母Phaffia rhodozyma細胞壁に対する溶解酵素のスクリーニングとアスタキサンチンの抽出

概要

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Screening was conducted for a lytic enzyme-producing microorganism with the aim of achieving efficient extraction of astaxanthin from Phaffia rhodozyma cells. The actinomycete Streptomyces rochei strain PHA-34 was found to produce the lytic enzyme, which mainly contained β-1,3 glucan-and β-1,6 glucan-hydrolyzing activities and which was stable within a wide pH range. The enzyme was induced by the addition of Phaffia cell wall into the culture broth. It was capable of lysing not only P. rhodozyma cells but also ascomycete and imperfecti yeasts in the logarithmic phase, as well as many basidiomycete yeasts regardless of the growth phase. Using the enzyme from S. rochsi PHA-34,the extraction of astaxanthin was superior in yield to that obtained by physical or chemical treatments or by using other commercially available lytic enzymes. The astaxanthin obtained using the PHA-34 enzyme was similar to synthetic astaxanthin on high performance liquid and thin layer chromatographies.
社団法人日本生物工学会の論文
1997-07-25