微生物による粘質多糖類の生産に関する研究 : (第3報)Bacillus strain F-573の生産する多糖類の構成糖について

概要

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D-Glucose, galactose and glucuronic acid were estimated as components in sulfuric acid-hydrolyzate of F 573 polysaccharide by various color reactions and paper chromatography. Identification of these monosaccharides was conducted as follows. Glucose was identified by its sepecific UV absorption spectra of chromogen in the glucostat. Similarly, glucuronic acid was defined by the thioglycolic acid-mannose-sulfuric acid reaction. A galactose fraction, which was isolated by cellulose column chromatography, was identified as α-methylphenyl-hydrazone derivative of the sugar. Subsequently, gas chromatography was applied to determine the ratio of the constituents of F 573 polysaccharide. Periodate oxidation was carried out and the results obtained were discussed.
社団法人日本生物工学会の論文
1971-01-25