窒素固定菌に関する研究(第2報) : 窒素固定に及ぼす2,3の要因について

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Two strains, A-400 and W-290,being isolated from the soil and the mud of Aichi-Kenk, Japan were the nitrogten-fixing microorganisms used in studying the efffects of some environmental factors on nitrogen fixation. They were identified to be the species Azotobacter.(1) Both strains could fix 12mgN/g glucose and 13mgN/g sucrose. The maximum amount of nitrogen fixed by A-400 was 840μg/ml, and taht by W-290 was 460μg/ml.(2) The efficiency of nitrogen fixation decreased with the increased concentration of substrate in the medium, and increased more in a stationary culture than in a shaken culture. The amount of nitrogen fixed by only A-400 increased by the adding of 3% CaCO_3 to the medium.(3) A concentration of over 7% of substrate in the medium caused a long lag phase. (4) Optimum pH and temperature for nitrogen fixation by A-400 and W-290 were both 7.0 to 7.8,and 25° to 29℃.(5) Nitrogen fixation was inhibited by the presence of nitrate. After the combined nitrogen was exhausted the organisms fixed nitrogen in the presence of an excess of carbon sources.(6) Carbon sources suitable for nitrogen fixation of the two strains were glucose, fructose and sucrose. W-290 also could easily utilize mannitol and sorbitol.
社団法人日本生物工学会の論文
1963-09-15